Linked Life Data

2174809

Source:http://linkedlifedata.com/resource/pubmed/id/2174809

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1991-1-23
pubmed:abstractText
The suppressor of Hairy-wing [su(Hw)] locus encodes a zinc finger-containing protein that binds to specific sequences of the Drosophila gypsy element, mediating the mutagenic effects of this retrotransposon. We carried out a detailed analysis of the su(Hw)-gypsy interaction using various biochemical assays. DNase I footprinting delimits a 37-bp region in the coding strand of gypsy that is protected from digestion by the binding of the su(Hw) protein. Specific DNA contacts involved in the interaction were determined by methylation protection analysis and by missing nucleoside experiments using hydroxyl radical. Results from these experiments indicate that the su(Hw) protein binds to sequences homologous to the octamer motif, which is recognized by homeo box-containing proteins in mammalian organisms. Furthermore, two DNA bends present on both sides of the binding site in the absence of protein favor the strength of this interaction.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0890-9369
pubmed:author
pubmed:issnType
Print
pubmed:volume
4
pubmed:geneSymbol
su(Hw)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1505-15
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
DNA bending is a determinant of binding specificity for a Drosophila zinc finger protein.
pubmed:affiliation
Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't