| pubmed-article:21642594 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:21642594 | lifeskim:mentions | umls-concept:C0079613 | lld:lifeskim |
| pubmed-article:21642594 | lifeskim:mentions | umls-concept:C0167627 | lld:lifeskim |
| pubmed-article:21642594 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:21642594 | lifeskim:mentions | umls-concept:C1514468 | lld:lifeskim |
| pubmed-article:21642594 | lifeskim:mentions | umls-concept:C0079411 | lld:lifeskim |
| pubmed-article:21642594 | lifeskim:mentions | umls-concept:C1705938 | lld:lifeskim |
| pubmed-article:21642594 | lifeskim:mentions | umls-concept:C1527178 | lld:lifeskim |
| pubmed-article:21642594 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:21642594 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:21642594 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:21642594 | pubmed:dateCreated | 2011-7-29 | lld:pubmed |
| pubmed-article:21642594 | pubmed:abstractText | Viral and fungal infections remain a leading cause of mortality in patients after hematopoietic stem cell transplantation (HSCT). Adoptive transfer of multipathogen-specific T cells is promising in restoring immunity and thereby preventing and treating infections, but approaches are currently limited because of time-consuming and laborious procedures. Therefore, we investigated a new strategy to simultaneously select T cells specific for viral and fungal pathogens based on activation-dependent expression of CD154. Single- and multipathogen-specific T-cell lines with high specificity for adenovirus (AdV), Epstein-Barr virus (EBV), cytomegalovirus (CMV), Candida albicans, and/or Aspergillus fumigatus could be readily generated within 14 days irrespective of the precursor frequency. The T-cell lines responded reproducibly to endogenously processed antigen and specifically proliferated upon antigenic stimulation. Although isolation based on CD154 favors enrichment of CD4(+) T cells, AdV-, EBV- and CMV-specific CD8(+) T cells could be expanded and demonstrated lysis of target cells. Conversely, T cell-mediated alloreactivity was almost abrogated compared with the starting fraction. This selection and/or expansion strategy may form the basis for future adoptive immunotherapy trials in patients at risk for multiple infections and may be translated to other antigens. | lld:pubmed |
| pubmed-article:21642594 | pubmed:language | eng | lld:pubmed |
| pubmed-article:21642594 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21642594 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:21642594 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21642594 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21642594 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21642594 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:21642594 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:21642594 | pubmed:issn | 1528-0020 | lld:pubmed |
| pubmed-article:21642594 | pubmed:author | pubmed-author:EinseleHerman... | lld:pubmed |
| pubmed-article:21642594 | pubmed:author | pubmed-author:ToppMax SMS | lld:pubmed |
| pubmed-article:21642594 | pubmed:author | pubmed-author:KrappmannSven... | lld:pubmed |
| pubmed-article:21642594 | pubmed:author | pubmed-author:KhannaNinaN | lld:pubmed |
| pubmed-article:21642594 | pubmed:author | pubmed-author:BergesCarsten... | lld:pubmed |
| pubmed-article:21642594 | pubmed:author | pubmed-author:LuratiSarahS | lld:pubmed |
| pubmed-article:21642594 | pubmed:author | pubmed-author:StuehlerClaud... | lld:pubmed |
| pubmed-article:21642594 | pubmed:author | pubmed-author:ConradBarbara... | lld:pubmed |
| pubmed-article:21642594 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:21642594 | pubmed:day | 28 | lld:pubmed |
| pubmed-article:21642594 | pubmed:volume | 118 | lld:pubmed |
| pubmed-article:21642594 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:21642594 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:21642594 | pubmed:pagination | 1121-31 | lld:pubmed |
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| pubmed-article:21642594 | pubmed:meshHeading | pubmed-meshheading:21642594... | lld:pubmed |
| pubmed-article:21642594 | pubmed:year | 2011 | lld:pubmed |
| pubmed-article:21642594 | pubmed:articleTitle | Generation of a multipathogen-specific T-cell product for adoptive immunotherapy based on activation-dependent expression of CD154. | lld:pubmed |
| pubmed-article:21642594 | pubmed:affiliation | Department of Biomedicine and Division of Infectious Diseases and Hospital Epidemiology, University Hospital of Basel, Basel, Switzerland. | lld:pubmed |
| pubmed-article:21642594 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:21642594 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
