Source:http://linkedlifedata.com/resource/pubmed/id/21642594
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
2011-7-29
|
| pubmed:abstractText |
Viral and fungal infections remain a leading cause of mortality in patients after hematopoietic stem cell transplantation (HSCT). Adoptive transfer of multipathogen-specific T cells is promising in restoring immunity and thereby preventing and treating infections, but approaches are currently limited because of time-consuming and laborious procedures. Therefore, we investigated a new strategy to simultaneously select T cells specific for viral and fungal pathogens based on activation-dependent expression of CD154. Single- and multipathogen-specific T-cell lines with high specificity for adenovirus (AdV), Epstein-Barr virus (EBV), cytomegalovirus (CMV), Candida albicans, and/or Aspergillus fumigatus could be readily generated within 14 days irrespective of the precursor frequency. The T-cell lines responded reproducibly to endogenously processed antigen and specifically proliferated upon antigenic stimulation. Although isolation based on CD154 favors enrichment of CD4(+) T cells, AdV-, EBV- and CMV-specific CD8(+) T cells could be expanded and demonstrated lysis of target cells. Conversely, T cell-mediated alloreactivity was almost abrogated compared with the starting fraction. This selection and/or expansion strategy may form the basis for future adoptive immunotherapy trials in patients at risk for multiple infections and may be translated to other antigens.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
1528-0020
|
| pubmed:author | |
| pubmed:issnType |
Electronic
|
| pubmed:day |
28
|
| pubmed:volume |
118
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1121-31
|
| pubmed:meshHeading |
pubmed-meshheading:21642594-Adenoviridae,
pubmed-meshheading:21642594-Antigens, Fungal,
pubmed-meshheading:21642594-Antigens, Viral,
pubmed-meshheading:21642594-Aspergillus fumigatus,
pubmed-meshheading:21642594-CD40 Ligand,
pubmed-meshheading:21642594-Candida albicans,
pubmed-meshheading:21642594-Cell Line,
pubmed-meshheading:21642594-Cell Separation,
pubmed-meshheading:21642594-Cytomegalovirus,
pubmed-meshheading:21642594-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:21642594-Flow Cytometry,
pubmed-meshheading:21642594-Herpesvirus 4, Human,
pubmed-meshheading:21642594-Humans,
pubmed-meshheading:21642594-Immunophenotyping,
pubmed-meshheading:21642594-Immunotherapy, Adoptive,
pubmed-meshheading:21642594-Lymphocyte Activation,
pubmed-meshheading:21642594-Lymphocyte Culture Test, Mixed,
pubmed-meshheading:21642594-Mycoses,
pubmed-meshheading:21642594-T-Lymphocytes,
pubmed-meshheading:21642594-Virus Diseases
|
| pubmed:year |
2011
|
| pubmed:articleTitle |
Generation of a multipathogen-specific T-cell product for adoptive immunotherapy based on activation-dependent expression of CD154.
|
| pubmed:affiliation |
Department of Biomedicine and Division of Infectious Diseases and Hospital Epidemiology, University Hospital of Basel, Basel, Switzerland.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|