Linked Life Data

21279244

Source:http://linkedlifedata.com/resource/pubmed/id/21279244

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2011-3-15
pubmed:abstractText
A novel application of two-dimensional correlation analysis has been employed to filter (1)H NMR heparin spectra distinguishing acceptable natural variation and the presence of foreign species. Analysis of contaminated heparin samples, compared to a dataset of accepted heparin samples using two-dimensional correlation spectroscopic analysis of their 1-dimensional (1)H NMR spectra, allowed the spectral features of contaminants to be recovered with high sensitivity, without having to resort to more complicated NMR experiments. Contaminants, which exhibited features distinct from those of heparin and those with features normally hidden within the spectral mass of heparin could be distinguished readily. A heparin sample which had been pre-mixed with a known contaminant, oversulfated chondroitin sulfate (OSCS), was tested against the heparin reference library. It was possible to recover the (1)H NMR spectrum of the OSCS component through difference 2D-COS power spectrum analysis of as little as 0.25% (w/w) with ease, and of 2% (w/w) for more challenging contaminants, whose NMR signals fell under those of heparin. The approach shows great promise for the quality control of heparin and provides the basis for greatly improved regulatory control for the analysis of heparin, as well as other intrinsically heterogeneous and varied products.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1364-5528
pubmed:author
pubmed:copyrightInfo
© The Royal Society of Chemistry 2011
pubmed:issnType
Electronic
pubmed:day
7
pubmed:volume
136
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1390-8
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
High-sensitivity visualisation of contaminants in heparin samples by spectral filtering of 1H NMR spectra.
pubmed:affiliation
Istituto di Ricerche Chimiche e Biochimiche G. Ronzoni, Via Giuseppe Colombo, 81 Milano 20133, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't