Linked Life Data

20709941

Source:http://linkedlifedata.com/resource/pubmed/id/20709941

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2010-11-2
pubmed:abstractText
TRAIL is known to cause death in tumor cells, but physiological regulation of its activity remains poorly characterized. We demonstrate that BCR triggering sensitizes transformed centroblast-like BL cells and peripheral blood memory B cells to TRAIL-mediated apoptosis. The sensitization correlated with surface down-regulation and intracellular retention of TRAIL-R4, along with changes in the expression of several Bcl-2 protein family members. Although enhancing FAS-mediated cell death, CD40 activation protected B cells from TRAIL-induced apoptosis. Combination of Ig cross-linking with CD40 ligation did not prevent TRAIL-R4 down-regulation but induced changes in the mitochondria-regulated pathway of apoptosis that are known to be associated with resistance to TRAIL. Human CD5(+) B cells, presumably stimulated by reactivity to self without immunological help, exhibited very high ex vivo sensitivity to TRAIL. Our results define the first B-lymphocyte-specific physiological signal that increases cellular sensitivity to TRAIL. This may be important for our understanding of TRAIL involvement in the control of B cell responses and aid in designing TRAIL-based therapies for B cell lymphomas.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1938-3673
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
88
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
937-45
pubmed:meshHeading
pubmed-meshheading:20709941-Antibody Specificity, pubmed-meshheading:20709941-Antigens, CD40, pubmed-meshheading:20709941-Apoptosis, pubmed-meshheading:20709941-Apoptosis Regulatory Proteins, pubmed-meshheading:20709941-B-Lymphocyte Subsets, pubmed-meshheading:20709941-B-Lymphocytes, pubmed-meshheading:20709941-Cell Culture Techniques, pubmed-meshheading:20709941-Cell Line, pubmed-meshheading:20709941-Down-Regulation, pubmed-meshheading:20709941-Flow Cytometry, pubmed-meshheading:20709941-Gene Expression Regulation, pubmed-meshheading:20709941-Humans, pubmed-meshheading:20709941-Immunologic Memory, pubmed-meshheading:20709941-Receptors, Antigen, B-Cell, pubmed-meshheading:20709941-TNF-Related Apoptosis-Inducing Ligand, pubmed-meshheading:20709941-Tumor Necrosis Factor-alpha, pubmed-meshheading:20709941-bcl-X Protein
pubmed:year
2010
pubmed:articleTitle
B cell receptor triggering sensitizes human B cells to TRAIL-induced apoptosis.
pubmed:affiliation
Johns Hopkins University School of Medicine, 733 North Broadway, Baltimore, MD 21205, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't