2070826

pubmed:Citation

2

Single cell suspensions of human keratinocytes when seeded onto floating three-dimensional gels constructed with type I collagen form a tissue resembling epidermis. These morphogenetic events occur in a serum-free environment in the absence of fibroblasts. Light and transmission electron microscopy show that cells form a basal layer plus suprabasilar cell layers corresponding to the stratum spinosum, stratum granulosum, and stratum corneum. The suprabasilar keratinocyte layers show morphologies which resemble intact skin in which cells are connected by desmosomes and contain intermediate filaments and keratohyalin-fillagrin granules. The basal cell layer differs from skin in vivo in that there is no connection to a basement membrane via hemidesmosomes. Cells in the basal layers are polarized as evidenced by the secretion of type IV collagen, heparan sulfate proteoglycans, and laminin at the cell membrane interface with the collagen gel. These proteins are not organized into a cytological basement membrane. Bullous pemphigoid antigen, a protein component of hemidesmosomes, is synthesized by basal keratinocytes, but like the basement membrane proteins it is not incorporated into a definable cytological structure. Keratinocytes in the basal and suprabasilar layers also synthesize alpha 2 beta 1 integrins. The mechanisms of keratinocyte adhesion to the gel may be through the interactions of this cell surface receptor with laminin and type IV collagen synthesized by the cell and/or direct interactions between the receptor and type I collagen within the gel. This in vitro experimental system is a useful model for defining the molecular events which control the formation and turnover of basement membranes and the mechanisms by which keratinocytes adhere to type I collagen when sheets of keratinocytes are used clinically for wound coverage.

eng

IM

MEDLINE

0014-4827

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NLM

443-57

pubmed-meshheading:2070826-Adolescent, pubmed-meshheading:2070826-Adult, pubmed-meshheading:2070826-Autoantigens, pubmed-meshheading:2070826-Basement Membrane, pubmed-meshheading:2070826-Carrier Proteins, pubmed-meshheading:2070826-Cells, Cultured, pubmed-meshheading:2070826-Chondroitin Sulfate Proteoglycans, pubmed-meshheading:2070826-Collagen, pubmed-meshheading:2070826-Cytoskeletal Proteins, pubmed-meshheading:2070826-Epidermis, pubmed-meshheading:2070826-Female, pubmed-meshheading:2070826-Heparan Sulfate Proteoglycans, pubmed-meshheading:2070826-Heparitin Sulfate, pubmed-meshheading:2070826-Humans, pubmed-meshheading:2070826-Immunohistochemistry, pubmed-meshheading:2070826-Integrins, pubmed-meshheading:2070826-Keratinocytes, pubmed-meshheading:2070826-Laminin, pubmed-meshheading:2070826-Male, pubmed-meshheading:2070826-Microscopy, Electron, pubmed-meshheading:2070826-Middle Aged, pubmed-meshheading:2070826-Nerve Tissue Proteins, pubmed-meshheading:2070826-Non-Fibrillar Collagens

Human keratinocytes cultured on collagen gels form an epidermis which synthesizes bullous pemphigoid antigens and alpha 2 beta 1 integrins and secretes laminin, type IV collagen, and heparan sulfate proteoglycan at the basal cell surface.

Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't