Source:http://linkedlifedata.com/resource/pubmed/id/20673797
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| rdf:type | |
| lifeskim:mentions |
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umls-concept:C1883709
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| pubmed:issue |
7
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| pubmed:dateCreated |
2010-9-14
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| pubmed:abstractText |
To evaluate the effect of CD133(+) cells (endothelial progenitor cells) on the hypoxia-induced suppression of axonal growth of cortical neurons and the destruction of blood vessels (endothelial cells), we used anterograde axonal tracing and immunofluorescence in organ co-cultures of the cortex and the spinal cord from 3-day-old neonatal rats. CD133(+) cells prepared from human umbilical cord blood were added to the organ co-cultures after hypoxic insult, and axonal growth, vascular damage and apoptosis were evaluated. Anterograde axonal tracing with 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate was used to analyze axonal projections from the cortex to the spinal cord. Immunolabeling co-cultured tissues of the cortex and the spinal cord were used to investigate the effect of CD133(+) cells on the survival of blood vessels and apoptosis in the brain cortex. Hypoxia remarkably suppressed axonal growth in organ co-cultures of the cortex and the spinal cord, and this suppression was significantly restored by the addition of CD133(+) cells. CD133(+) cells also reduced the hypoxia-induced destruction of the cortical blood vessels and apoptosis. CD133(+) cells had protective effects on hypoxia-induced injury of neurons and blood vessels of the brain cortex in vitro. These results suggest that CD133(+) cell transplantation may be a possible therapeutic intervention for perinatal hypoxia-induced brain injury.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Nov
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| pubmed:issn |
1873-474X
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| pubmed:author |
pubmed-author:AsaharaTakayukiT,
pubmed-author:FujiwaraHisayaH,
pubmed-author:IshikawaMasakazuM,
pubmed-author:KameiNaosukeN,
pubmed-author:KudoYoshikiY,
pubmed-author:MiyoshiHiroshiH,
pubmed-author:NakamaeToshioT,
pubmed-author:OchiMitsuoM,
pubmed-author:TanakaNorifumiN,
pubmed-author:YamamotoRisakoR
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| pubmed:copyrightInfo |
Copyright © 2010 ISDN. Published by Elsevier Ltd. All rights reserved.
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| pubmed:issnType |
Electronic
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| pubmed:volume |
28
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
581-7
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| pubmed:meshHeading |
pubmed-meshheading:20673797-Animals,
pubmed-meshheading:20673797-Animals, Newborn,
pubmed-meshheading:20673797-Anoxia,
pubmed-meshheading:20673797-Antigens, CD,
pubmed-meshheading:20673797-Apoptosis,
pubmed-meshheading:20673797-Axons,
pubmed-meshheading:20673797-Cells, Cultured,
pubmed-meshheading:20673797-Cerebral Cortex,
pubmed-meshheading:20673797-Cerebrovascular Circulation,
pubmed-meshheading:20673797-Coculture Techniques,
pubmed-meshheading:20673797-Fetal Blood,
pubmed-meshheading:20673797-Glycoproteins,
pubmed-meshheading:20673797-Humans,
pubmed-meshheading:20673797-Peptides,
pubmed-meshheading:20673797-Rats,
pubmed-meshheading:20673797-Rats, Sprague-Dawley,
pubmed-meshheading:20673797-Stem Cells,
pubmed-meshheading:20673797-Tissue Culture Techniques
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| pubmed:year |
2010
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| pubmed:articleTitle |
CD133+ cells from human umbilical cord blood reduce cortical damage and promote axonal growth in neonatal rat organ co-cultures exposed to hypoxia.
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| pubmed:affiliation |
Department of Obstetrics and Gynecology, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima City, Hiroshima, 734-8551, Japan.
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| pubmed:publicationType |
Journal Article
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