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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2010-7-23
pubmed:abstractText
A solid phase microextraction--liquid chromatography with ultraviolet detection (SPME-LC-UV) method for the determination of the antimicrobial agent chloramphenicol was developed. The performances of three commercially available fibers were compared; the Carbowax/TPR-100 was found to provide the most efficient extraction. All the aspects influencing the fiber adsorption (extraction time, temperature, pH, salt addition) and desorption (desorption and injection time, desorption solvent mixture composition) of the analyte were investigated. The method was eventually applied to the determination of the drug in both biological (urine) and environmental (tap and sea water) samples. The optimized procedure required a simple sample pretreatment, isocratic elution, and provided enough sensitivity for the analyte determination in the considered samples. The investigated linear ranges were 37-1000 ng/ml (urine), 0.1-10 ng/ml (tap water), 0.3-30 ng/ml (sea water). Within-day and between-days RSD% ranged between 5.5-6.2 and 8.7-9.0 (urine), 5.1-6.0 and 8.4-8.8 (tap water), 5.4-5.7 and 8.6-8.9 (sea water). Estimated LOD and LOQ were 37 and 95 ng/ml (urine), 0.1 and 0.3 ng/ml (tap water), 0.3 and 0.7 ng/ml (sea water).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1873-264X
pubmed:author
pubmed:issnType
Electronic
pubmed:day
2
pubmed:volume
53
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
440-4
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Solid phase microextraction--liquid chromatography (SPME-LC) determination of chloramphenicol in urine and environmental water samples.
pubmed:affiliation
Dipartimento di Chimica, Università degli Studi di Bari, Via Orabona 4, 70126 Bari, Italy.
pubmed:publicationType
Journal Article