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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
2010-5-13
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pubmed:abstractText |
We produced low-molecular-weight heparin/protamine nanoparticles (LMW-H/P NPs) as a carrier for heparin-binding growth factors, such as fibroblast growth factor-2 (FGF-2). A mixture of low-molecular-weight heparin (MW: about 5000 Da, 6.4 mg/mL) and protamine (MW: about 3000 Da, 10 mg/mL) at a ratio of 7:3 (vol:vol) yields a dispersion of microparticles (1-6 microm in diameter). In this study, diluted low-molecular-weight heparin solution in saline (0.32 mg/mL) mixed with diluted protamine (0.5 mg/mL) at a ratio at 7:3 (vol:vol) resulted in soluble nanoparticles (112.5 +/- 46.1 nm in diameter). The generated NPs could be then stabilized by adding 2 mg/mL dextran (MW: 178-217 kDa) and remained soluble after lyophilization of dialyzed LMW-H/P NP solution. We then evaluated the capacity of LMW-H/P NPs to protect activity of FGF-2. Interaction between FGF-2 and LMW-H/P NPs substantially prolonged the biological half-life of FGF-2. Furthermore, FGF-2 molecules were protected from inactivation by heat and proteolysis in the presence of LMW-H/P NPs.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/20463930-10362840,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20463930-11157643,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20463930-12753748,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20463930-12842774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20463930-14607508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20463930-15603823,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20463930-16673389,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20463930-17806115,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20463930-19051304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20463930-19074504,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20463930-3528177,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20463930-7667826,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20463930-8073404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20463930-8836040,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20463930-9388109
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1178-2013
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
147-55
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pubmed:dateRevised |
2010-9-30
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pubmed:meshHeading |
pubmed-meshheading:20463930-Cells, Cultured,
pubmed-meshheading:20463930-Crystallization,
pubmed-meshheading:20463930-Diffusion,
pubmed-meshheading:20463930-Drug Carriers,
pubmed-meshheading:20463930-Drug Compounding,
pubmed-meshheading:20463930-Endothelial Cells,
pubmed-meshheading:20463930-Fibroblast Growth Factor 2,
pubmed-meshheading:20463930-Heparin, Low-Molecular-Weight,
pubmed-meshheading:20463930-Humans,
pubmed-meshheading:20463930-Macromolecular Substances,
pubmed-meshheading:20463930-Materials Testing,
pubmed-meshheading:20463930-Metabolic Clearance Rate,
pubmed-meshheading:20463930-Molecular Conformation,
pubmed-meshheading:20463930-Nanomedicine,
pubmed-meshheading:20463930-Nanostructures,
pubmed-meshheading:20463930-Particle Size,
pubmed-meshheading:20463930-Protamines,
pubmed-meshheading:20463930-Surface Properties
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pubmed:year |
2010
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pubmed:articleTitle |
Preparation and characterization of low-molecular-weight heparin/protamine nanoparticles (LMW-H/P NPs) as FGF-2 carrier.
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pubmed:affiliation |
Research Institute, National Defense Medical College, Tokorozawa, Saitama, Japan.
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pubmed:publicationType |
Journal Article
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