Source:http://linkedlifedata.com/resource/pubmed/id/20386499
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
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| pubmed:dateCreated |
2010-10-26
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| pubmed:abstractText |
In this study, we examined the effects of direct hemoperfusion through filters with immobilized polymyxin B (PMX-DHP) on leukocyte function and plasma levels of cytokines in patients with septic shock. We found that PMX-DHP caused increased expression of C-X-C chemokine receptor 1 (CXCR1) and CXCR2, along with decreased expression of CD64 and CD11b, by circulating neutrophils in patients with septic shock. Plasma levels of cytokines, including interleukin 6 (IL-6), IL-8, IL-10, and high-mobility group box 1, were elevated in patients with septic shock compared with healthy controls, but cytokine levels were not altered by PMX-DHP. These results suggest that PMX-DHP influences neutrophils via a mechanism that does not involve cytokine. Ex vivo perfusion of heparinized blood from patients with sepsis and septic shock through PMX filters in a laboratory circuit caused a significant decrease in neutrophil and monocyte counts. After 120 min of perfusion, neutrophils, monocytes, and lymphocytes were decreased by 78%, 70%, and 10%, respectively, compared with baseline values. Flow cytometric analysis indicated that activated neutrophils with high levels of CD11b/CD64 expression and low levels of CXCR1/CXCR2 expression showed preferential adhesion to PMX filters. Neutrophils isolated from the blood after ex vivo PMX perfusion caused less damage to an endothelial cell monolayer than cells from sham-treated blood, whereas neutrophil phagocytosis of opsonized Escherichia coli was unaffected. These results indicate that PMX-DHP selectively removes activated neutrophils and reduces the ability of circulating cells to cause endothelial damage. Selective removal of activated neutrophils using PMX-DHP may improve the systemic inflammatory response in patients with septic shock.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/HMGB1 Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Opsonin Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Polymyxin B
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| pubmed:status |
MEDLINE
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| pubmed:month |
Nov
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| pubmed:issn |
1540-0514
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
34
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
461-6
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| pubmed:meshHeading |
pubmed-meshheading:20386499-Acute Lung Injury,
pubmed-meshheading:20386499-Aged,
pubmed-meshheading:20386499-Antigens, Surface,
pubmed-meshheading:20386499-Cells, Cultured,
pubmed-meshheading:20386499-Cytokines,
pubmed-meshheading:20386499-Endothelial Cells,
pubmed-meshheading:20386499-Escherichia coli,
pubmed-meshheading:20386499-Female,
pubmed-meshheading:20386499-Filtration,
pubmed-meshheading:20386499-Flow Cytometry,
pubmed-meshheading:20386499-HMGB1 Protein,
pubmed-meshheading:20386499-Hemoperfusion,
pubmed-meshheading:20386499-Humans,
pubmed-meshheading:20386499-Leukapheresis,
pubmed-meshheading:20386499-Leukocyte Count,
pubmed-meshheading:20386499-Leukocytes,
pubmed-meshheading:20386499-Lymphocyte Activation,
pubmed-meshheading:20386499-Male,
pubmed-meshheading:20386499-Middle Aged,
pubmed-meshheading:20386499-Neutrophils,
pubmed-meshheading:20386499-Opsonin Proteins,
pubmed-meshheading:20386499-Phagocytosis,
pubmed-meshheading:20386499-Polymyxin B,
pubmed-meshheading:20386499-Shock, Septic
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| pubmed:year |
2010
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| pubmed:articleTitle |
Apheresis of activated leukocytes with an immobilized polymyxin B filter in patients with septic shock.
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| pubmed:affiliation |
Department of Emergency & Critical Care Medicine, Aichi Medical University, Nagakute, Aichi, Japan. kuma@aichi-med-u.ac.jp
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| pubmed:publicationType |
Journal Article,
Clinical Trial
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