Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1991-5-31
pubmed:abstractText
A posttranslational modification site in natural and recombinant urinary-type plasminogen activators (urokinases; EC 3.4.21.31) has been localized to Thr-18, in the growth factor domain of the molecule. This is the region of urinary plasminogen activator responsible for its specific receptor binding. An unusual carbohydrate-protein linkage, a single monosaccharide, fucose, covalently attached directly to threonine in the peptide, is described here. The glycan moiety and the site of modification have been identified with mass spectrometry and confirmed by carbohydrate composition analysis, Edman degradation, and one- and two-dimensional NMR studies. This type of modification is normally not detected without mass spectrometry because the fucose-threonine bond is hydrolyzed under standard acidic conditions of the amino acid analysis and Edman sequencing. This modification may be widely found in other proteins.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2023947-1141232, http://linkedlifedata.com/resource/pubmed/commentcorrection/2023947-2393398, http://linkedlifedata.com/resource/pubmed/commentcorrection/2023947-2930999, http://linkedlifedata.com/resource/pubmed/commentcorrection/2023947-3003076, http://linkedlifedata.com/resource/pubmed/commentcorrection/2023947-3031025, http://linkedlifedata.com/resource/pubmed/commentcorrection/2023947-3063136, http://linkedlifedata.com/resource/pubmed/commentcorrection/2023947-3127884, http://linkedlifedata.com/resource/pubmed/commentcorrection/2023947-3144052, http://linkedlifedata.com/resource/pubmed/commentcorrection/2023947-3389500, http://linkedlifedata.com/resource/pubmed/commentcorrection/2023947-3977832, http://linkedlifedata.com/resource/pubmed/commentcorrection/2023947-6338833, http://linkedlifedata.com/resource/pubmed/commentcorrection/2023947-6606428, http://linkedlifedata.com/resource/pubmed/commentcorrection/2023947-6661238, http://linkedlifedata.com/resource/pubmed/commentcorrection/2023947-6754569, http://linkedlifedata.com/resource/pubmed/commentcorrection/2023947-6754572, http://linkedlifedata.com/resource/pubmed/commentcorrection/2023947-6791580, http://linkedlifedata.com/resource/pubmed/commentcorrection/2023947-7263634
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
88
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3992-6
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Characterization of a posttranslational fucosylation in the growth factor domain of urinary plasminogen activator.
pubmed:affiliation
Analytical Research Department, Abbott Laboratories, North Chicago, IL 60064.
pubmed:publicationType
Journal Article