Source:http://linkedlifedata.com/resource/pubmed/id/20051473
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
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| pubmed:dateCreated |
2010-4-30
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| pubmed:abstractText |
Macrophages are involved in many aspects of development, host defense, pathology, and homeostasis. Their normal differentiation, proliferation, and survival are controlled by CSF-1 via the activation of the CSF1R. A recently discovered cytokine, IL-34, was shown to bind the same receptor in humans. Chicken is a widely used model organism in developmental biology, but the factors that control avian myelopoiesis have not been identified previously. The CSF-1, IL-34, and CSF1R genes in chicken and zebra finch were identified from respective genomic/cDNA sequence resources. Comparative analysis of the avian CSF1R loci revealed likely orthologs of mammalian macrophage-specific promoters and enhancers, and the CSF1R gene is expressed in the developing chick embryo in a pattern consistent with macrophage-specific expression. Chicken CSF-1 and IL-34 were expressed in HEK293 cells and shown to elicit macrophage growth from chicken BM cells in culture. Comparative sequence and co-evolution analysis across all vertebrates suggests that the two ligands interact with distinct regions of the CSF1R. These studies demonstrate that there are two separate ligands for a functional CSF1R across all vertebrates.
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| pubmed:grant | |
| pubmed:commentsCorrections | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Interleukins,
http://linkedlifedata.com/resource/pubmed/chemical/Macrophage Colony-Stimulating Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Macrophage...,
http://linkedlifedata.com/resource/pubmed/chemical/interleukin-34, human
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| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
1938-3673
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
87
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
753-64
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| pubmed:dateRevised |
2010-11-18
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| pubmed:meshHeading |
pubmed-meshheading:20051473-Amino Acid Sequence,
pubmed-meshheading:20051473-Animals,
pubmed-meshheading:20051473-Biological Evolution,
pubmed-meshheading:20051473-Birds,
pubmed-meshheading:20051473-Chick Embryo,
pubmed-meshheading:20051473-Chickens,
pubmed-meshheading:20051473-Conserved Sequence,
pubmed-meshheading:20051473-Finches,
pubmed-meshheading:20051473-Gene Expression,
pubmed-meshheading:20051473-Humans,
pubmed-meshheading:20051473-In Situ Hybridization,
pubmed-meshheading:20051473-Interleukins,
pubmed-meshheading:20051473-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:20051473-Macrophages,
pubmed-meshheading:20051473-Molecular Sequence Data,
pubmed-meshheading:20051473-Phylogeny,
pubmed-meshheading:20051473-Protein Structure, Quaternary,
pubmed-meshheading:20051473-Receptor, Macrophage Colony-Stimulating Factor,
pubmed-meshheading:20051473-Sequence Homology, Amino Acid,
pubmed-meshheading:20051473-Structural Homology, Protein
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| pubmed:year |
2010
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| pubmed:articleTitle |
Pivotal Advance: Avian colony-stimulating factor 1 (CSF-1), interleukin-34 (IL-34), and CSF-1 receptor genes and gene products.
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| pubmed:affiliation |
The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Roslin, United Kingdom.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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