Linked Life Data

20018269

Source:http://linkedlifedata.com/resource/pubmed/id/20018269

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2010-2-15
pubmed:abstractText
Toxoplasma gondii is a protozoan parasite causing toxoplasmosis to almost one-third of population all over the world. One of the most efficient ways to control this disease is immunization. However, so far, there is no effective vaccine available against this pathogen. Recently, a baculovirus pseudotype with vesicular stomatitis virus G protein (Bac-VSV-G) was found to efficiently transduce and express transgenes on mammalian cells, so it was considered as an excellent expressing vector. In this study, the value of Bac-VSV-G in delivering T. gondii antigen was investigated. T. gondii SAG1 gene was cloned into Bac-VSV-G, and recombinant baculovirus BV-G-SAG1 was obtained. Indirect immunofluorescence test showed BV-G-SAG1 was efficiently transduced and expressed in pig kidney cells. Then BALB/c mice were immunized with BV-G-SAG1 at different doses (1 x 10(8), 1 x 10(9), and 1 x 10(10)PFU/mouse) and challenged with T. gondii RH strain tachyzoites after immunization. The levels of specific T. gondii antibody, interferon (IFN)-gamma, IL-4, IL-10 expression and release, and the survival rate of treated mice were evaluated. Compared with the mice immunized with DNA vaccine (pcDNA/SAG1) encoding the same gene, BV-G-SAG1 induced higher levels of specific T. gondii antibody and (IFN)-gamma expression with dose-dependent manner and the survival rate of mice with BV-G-SAG1 was significantly improved. These results indicated that pseudotype baculovirus-mediated gene delivery can be utilized as an alternative strategy to develop new generation of vaccines against T. gondii infection.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1873-2518
pubmed:author
pubmed:copyrightInfo
Copyright (c) 2009 Elsevier Ltd. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
17
pubmed:volume
28
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1803-7
pubmed:meshHeading
pubmed-meshheading:20018269-Animals, pubmed-meshheading:20018269-Antibodies, Protozoan, pubmed-meshheading:20018269-Antigens, Protozoan, pubmed-meshheading:20018269-Baculoviridae, pubmed-meshheading:20018269-Cell Line, pubmed-meshheading:20018269-Cell Proliferation, pubmed-meshheading:20018269-Female, pubmed-meshheading:20018269-Interferon-gamma, pubmed-meshheading:20018269-Interleukin-10, pubmed-meshheading:20018269-Interleukin-4, pubmed-meshheading:20018269-Lymphocyte Activation, pubmed-meshheading:20018269-Mice, pubmed-meshheading:20018269-Mice, Inbred BALB C, pubmed-meshheading:20018269-Protozoan Proteins, pubmed-meshheading:20018269-Protozoan Vaccines, pubmed-meshheading:20018269-Spleen, pubmed-meshheading:20018269-Swine, pubmed-meshheading:20018269-Toxoplasma, pubmed-meshheading:20018269-Toxoplasmosis, Animal, pubmed-meshheading:20018269-Vaccines, DNA
pubmed:year
2010
pubmed:articleTitle
Construction and immunogenicity of pseudotype baculovirus expressing Toxoplasma gondii SAG1 protein in BALB/c mice model.
pubmed:affiliation
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't