Linked Life Data

1996674

Source:http://linkedlifedata.com/resource/pubmed/id/1996674

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2 Pt 2
pubmed:dateCreated
1991-3-22
pubmed:abstractText
Nephrocalcin (NC), a urinary calcium oxalate monohydrate (COM) crystal growth inhibitor, was purified from kidneys of nine vertebrate species including humans. All isolates were glycoproteins with high contents of aspartic and glutamic acids and small amounts of basic and aromatic amino acids. Carbohydrate contents ranged from 4 to 16 wt% among the different species, but all contained fucose, mannose, galactose, glucose, galactosamine, glucosamine, and N-acetylneuraminic acid. Although amino acid and carbohydrate compositions were similar, dissociation constants derived from calcium oxalate monohydrate inhibition varied between 10(-7) and 10(-8) M, and highest affinity could be related to highest ability of the kidney of origin to concentrate urine. Using an antibody raised against NC from human kidney tissue culture medium, we found strong immunoreactivity with two species, pigs and sheep. Thus far all vertebrate kidneys possess COM growth-inhibiting material that seems to be of glycoprotein character, and all the glycoproteins isolated so far share similar features, suggesting NC-like proteins are a well-conserved trait.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0002-9513
pubmed:author
pubmed:issnType
Print
pubmed:volume
260
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
F243-8
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Isolation of nephrocalcin from kidney tissue of nine vertebrate species.
pubmed:affiliation
Nephrology Program, University of Chicago, Illinois 60637.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.