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rdf:type |
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lifeskim:mentions |
umls-concept:C0001675,
umls-concept:C0003250,
umls-concept:C0040300,
umls-concept:C0043457,
umls-concept:C0205409,
umls-concept:C0220908,
umls-concept:C0243092,
umls-concept:C1167624,
umls-concept:C1548779,
umls-concept:C1947902,
umls-concept:C2827499
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pubmed:issue |
1-2
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pubmed:dateCreated |
2009-6-29
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pubmed:abstractText |
The lack of a sufficient number of antibodies represents an obstacle in the research performed using the zebrafish (Danio rerio) as a model organism. On the other hand, high-throughput generation of antibodies, especially those suitable for immunohistochemistry, is not an established methodology. Here we present the results of an immunization experiment with a zebrafish tissue lysate that allowed for the isolation of hundreds of monoclonal antibodies suitable for labeling of a large variety of zebrafish tissue and cell structures. Some of them were further characterized in terms of detailed localization and age-dependent expression. In addition, the antigen recognized by one of them was first immunoprecipitated and then identified by mass spectrometry. Furthermore, immunofluorescence-competent recombinant antibodies were also isolated by panning large repertoire phage display libraries, in both single-chain (scFv) and single-domain (VHH) format. Such selection alternative is simpler to organize and could contribute to limit the costs of antibody screening and production.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1872-7905
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
31
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pubmed:volume |
346
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9-17
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pubmed:meshHeading |
pubmed-meshheading:19410577-Age Factors,
pubmed-meshheading:19410577-Animals,
pubmed-meshheading:19410577-Antibodies, Monoclonal,
pubmed-meshheading:19410577-Blotting, Western,
pubmed-meshheading:19410577-Epitope Mapping,
pubmed-meshheading:19410577-Hybridomas,
pubmed-meshheading:19410577-Immunization,
pubmed-meshheading:19410577-Immunoglobulin Heavy Chains,
pubmed-meshheading:19410577-Immunoglobulin Variable Region,
pubmed-meshheading:19410577-Immunohistochemistry,
pubmed-meshheading:19410577-Immunoprecipitation,
pubmed-meshheading:19410577-Mice,
pubmed-meshheading:19410577-Mice, Inbred BALB C,
pubmed-meshheading:19410577-Microscopy, Fluorescence,
pubmed-meshheading:19410577-Peptide Library,
pubmed-meshheading:19410577-Recombinant Proteins,
pubmed-meshheading:19410577-Zebrafish,
pubmed-meshheading:19410577-Zebrafish Proteins
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pubmed:year |
2009
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pubmed:articleTitle |
Monoclonal antibodies isolated by large-scale screening are suitable for labeling adult zebrafish (Danio rerio) tissues and cell structures.
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pubmed:affiliation |
Fondazione IFOM (Istituto FIRC di Oncologia Molecolare), Milan, Italy.
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pubmed:publicationType |
Journal Article
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