Source:http://linkedlifedata.com/resource/pubmed/id/19192342
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2009-2-4
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| pubmed:abstractText |
The aim of the current study was to evaluate whether near-infrared (NIR) spectroscopy-generated metabolomic data obtained from oocyte culture samples would correlate with nuclear maturity status and derived embryo development. A total of 412 oocyte culture samples were collected from 43 patient cycles. Metabolomic profiles of metaphase I and II oocytes were obtained by NIR spectroscopy and were significantly different from each other and from profiles of prophase I (germinal vesicle) oocytes (P +/- 0.001 at the 95% confidence interval). Additionally, NIR spectroscopic analysis of culture medium of oocytes that developed to grade A embryos on day 3 demonstrated significantly higher viability indices (0.62 +/- 0.23) than those that developed to grades C/D (0.42 +/- 0.26; P < 0.006); and on day 5 grade A (0.37 +/- 0.20) was also higher than grades C/D (0.14 +/- 0.21; P < 0.02). Metabolomic profiles of oocytes that resulted in pregnancy had higher viability indices (0.87 +/- 0.27) than those that did not (0.44 +/- 0.17; P < 0.0001). The results of the current study demonstrate that metabolomic profiling from spent culture medium of the oocyte is related to nuclear maturity, is able to predict embryo development at day 3 and day 5 stages, and relates to embryo viability.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Feb
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| pubmed:issn |
1472-6491
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
18
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
219-25
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| pubmed:meshHeading |
pubmed-meshheading:19192342-Adult,
pubmed-meshheading:19192342-Biological Markers,
pubmed-meshheading:19192342-Cell Survival,
pubmed-meshheading:19192342-Cells, Cultured,
pubmed-meshheading:19192342-Culture Media,
pubmed-meshheading:19192342-Embryo Transfer,
pubmed-meshheading:19192342-Female,
pubmed-meshheading:19192342-Humans,
pubmed-meshheading:19192342-Male,
pubmed-meshheading:19192342-Metabolomics,
pubmed-meshheading:19192342-Oocytes,
pubmed-meshheading:19192342-Pregnancy,
pubmed-meshheading:19192342-Pregnancy Rate,
pubmed-meshheading:19192342-Quality Control,
pubmed-meshheading:19192342-Research Design,
pubmed-meshheading:19192342-Sensitivity and Specificity,
pubmed-meshheading:19192342-Spectroscopy, Near-Infrared,
pubmed-meshheading:19192342-Sperm Injections, Intracytoplasmic
|
| pubmed:year |
2009
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| pubmed:articleTitle |
Metabolomic assessment of oocyte viability.
|
| pubmed:affiliation |
Reproductive Biology Associates, Assisted Reproduction, 1150 Lake Hearn Drive, Suite 600, Atlanta, GA 30342, USA. peter.nagy@rba-online.com
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| pubmed:publicationType |
Journal Article,
Clinical Trial
|