Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2008-12-8
pubmed:abstractText
Epibatidine (EPI), a potent cholinergic agonist, disrupts acetylcholine-dependent spontaneous retinal activity. Early patch-clamp recordings in juvenile ferrets suggested that EPI blocks all retinal ganglion cell (RGC) action potentials when applied to the retina. In contrast, recent experiments on the developing mouse that relied on multielectrode array (MEA) recordings reported that EPI application decorrelates the activity of neighboring RGCs and eliminates retinal waves while preserving the spiking activity of many neurons. The different techniques used in previous studies raise the question of whether EPI has different effects on RGC activity in mouse compared with that in ferret. A resolution of this issue is essential for interpreting the results of developmental studies that relied on EPI to manipulate retinal activity. Our goal was to compare the effects of EPI on the spontaneous discharges of RGCs in mouse and ferret using 60-electrode MEA as well as patch-clamp recordings during the developmental stage when retinal waves are driven by acetylcholine in both species. We found that in both mouse and ferret EPI decorrelates RGC activity and eliminates retinal waves. However, EPI does not block all spontaneous activity in either species. Instead, our whole cell recordings reveal that EPI silences more than half of all RGCs while significantly increasing the activity of the remainder. These results have important implications for interpreting the results of previous studies that relied on this cholinergic agonist to perturb retinal activity.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-10202531, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-10349970, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-10595518, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-10627612, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-10964962, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-11027228, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-11044727, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-11153654, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-11347820, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-11404416, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-12417667, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-12436420, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-12533612, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-12722106, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-12906941, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-12917368, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-14687550, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-15213299, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-15308679, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-15572115, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-15608630, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-15820390, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-15978026, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-16025107, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-16033903, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-16129735, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-16337917, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-16462736, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-16687510, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-17009926, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-17046688, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-17254766, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-17341130, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-17433447, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-17597586, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-17715349, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-18558864, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-18701068, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-2035024, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-2320593, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-3175636, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-8240814, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-8638165, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-8895456, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-9516112, http://linkedlifedata.com/resource/pubmed/commentcorrection/18922954-9570807
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0022-3077
pubmed:author
pubmed:issnType
Print
pubmed:volume
100
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3253-63
pubmed:dateRevised
2010-12-3
pubmed:meshHeading
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