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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1991-5-20
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pubmed:abstractText |
A molecular genetic analysis has been combined with an in vitro biochemical approach to define the functional interactions required for nucleotidyl protein formation during poliovirus RNA synthesis. A site-directed lesion into the hydrophobic domain of a viral membrane protein produced a mutant virus that is defective in RNA synthesis at 39 degrees C. The phenotypic expression of this lesion affects initiation of RNA synthesis, in vitro uridylylation of the genome-linked protein (VPg), and the in vivo synthesis of plus-strand viral RNAs. Our results support a model that employs a viral membrane protein as carrier for VPg in the initiation of plus-strand RNA synthesis. Our data also suggest that a separate mechanism could be used in the initiation of minus-strand RNA synthesis, thereby providing a means for strand-specific regulation of picornavirus RNA replication.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1850038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1850038-191841,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1850038-196204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1850038-198796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1850038-211265,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1850038-2159557,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1850038-2170027,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1850038-219230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1850038-2417240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1850038-2422394,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1850038-2536107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1850038-2553272,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1850038-2829191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1850038-2839711,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1850038-2845132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1850038-2985794,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1850038-3018300,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1850038-3027407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1850038-3029989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1850038-3039171,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1850038-4318974,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1850038-4373729,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1850038-4705382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1850038-6244109,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1850038-6252335,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1850038-6277514,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1850038-6291782,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1850038-6308897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1850038-6312101,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1850038-6323750,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1850038-6324172,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1850038-6324173
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
65
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2647-54
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1850038-Amino Acid Sequence,
pubmed-meshheading:1850038-DNA, Viral,
pubmed-meshheading:1850038-Gene Expression Regulation, Viral,
pubmed-meshheading:1850038-Kinetics,
pubmed-meshheading:1850038-Molecular Sequence Data,
pubmed-meshheading:1850038-Mutation,
pubmed-meshheading:1850038-Phenotype,
pubmed-meshheading:1850038-Poliovirus,
pubmed-meshheading:1850038-RNA, Viral,
pubmed-meshheading:1850038-Temperature,
pubmed-meshheading:1850038-Viral Envelope Proteins
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pubmed:year |
1991
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pubmed:articleTitle |
Role of a viral membrane polypeptide in strand-specific initiation of poliovirus RNA synthesis.
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pubmed:affiliation |
Department of Microbiology and Molecular Genetics, College of Medicine, University of California, Irvine 92717.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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