Linked Life Data

18414498

Source:http://linkedlifedata.com/resource/pubmed/id/18414498

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2008-7-25
pubmed:abstractText
This study explores the neuroprotective action of tumor necrosis factor-alpha (TNF-alpha) induced during physical exercise, which, consequently, reduces matrix metalloproteinase-9 (MMP-9) activity and ameliorates blood-brain barrier (BBB) dysfunction in association with extracellular signal-regulated kinase 1 and 2 (ERK1/2) phosphorylation. Adult male Sprague-Dawley rats were subjected to exercise on a treadmill for 3 weeks. A 2-h middle cerebral artery occlusion and reperfusion was administered to exercised and nonexercised animals to induce stroke. Exercised ischemic rats were subjected to TNF-alpha inhibition and ERK1/2 by TNF-alpha antibody or UO126. Nissl staining of coronal sections revealed the infarct volume. Evans blue extravasation and water content evaluated BBB function. Western blot was performed to analyze protein expression of TNF-alpha, ERK1/2, phosphorylated ERK1/2, the basal laminar protein collagen IV, and MMP-9. The activity of MMP-9 was determined by gelatin zymography. Tumor necrosis factor-alpha expression and ERK1/2 phosphorylation were upregulated during exercise. Infarct volume, brain edema, and Evans blue extravasation all significantly decreased in exercised ischemic rats. Collagen IV production increased in exercised rats and remained high after stroke, whereas MMP-9 protein level and activity decreased. These results were negated and returned toward nonexercised values once TNF-alpha or ERK1/2 was blocked. We concluded that preischemic, exercise-induced TNF-alpha markedly decreases BBB dysfunction by using the ERK1/2 pathway.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1559-7016
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
28
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1422-30
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:18414498-Animals, pubmed-meshheading:18414498-Antibodies, pubmed-meshheading:18414498-Blood-Brain Barrier, pubmed-meshheading:18414498-Butadienes, pubmed-meshheading:18414498-Collagen Type IV, pubmed-meshheading:18414498-Corpus Callosum, pubmed-meshheading:18414498-Enzyme Inhibitors, pubmed-meshheading:18414498-Male, pubmed-meshheading:18414498-Matrix Metalloproteinase 9, pubmed-meshheading:18414498-Mitogen-Activated Protein Kinase 1, pubmed-meshheading:18414498-Mitogen-Activated Protein Kinase 3, pubmed-meshheading:18414498-Nitriles, pubmed-meshheading:18414498-Phosphorylation, pubmed-meshheading:18414498-Physical Conditioning, Animal, pubmed-meshheading:18414498-Rats, pubmed-meshheading:18414498-Rats, Sprague-Dawley, pubmed-meshheading:18414498-Stroke, pubmed-meshheading:18414498-Tumor Necrosis Factor-alpha, pubmed-meshheading:18414498-Up-Regulation
pubmed:year
2008
pubmed:articleTitle
Preischemic induction of TNF-alpha by physical exercise reduces blood-brain barrier dysfunction in stroke.
pubmed:affiliation
Department of Neurosurgery, The University of Texas Health Science Center at San Antonio, San Antonio, Texas 78229-3900, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't