Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-2-21
pubmed:abstractText
Human plasma is regarded the most complex and well-known clinical specimen that can be easily obtained; alterations in the levels of plasma proteins or their corresponding enzyme activities may reflect either a healthy or a diseased state. Given that there is no defined genomic information as to the intact protein components in plasma, protein profiling could be the first step toward its molecular characterization. Several problems exist in the analysis of plasma proteins, however. For example, the widest dynamic range of protein concentrations, the presence of high-abundance proteins, and post-translational modifications need to be considered before proteomic studies are undertaken. In particular, efficient depletion or pre-fractionation of high-abundance proteins is crucial for the identification of low-abundance proteins that may contain potential biomarkers. After the removal of high-abundance proteins, protein profiling can be initiated using two-dimensional electrophoresis (2DE), which has been widely used for displaying the differential proteome under specific physiological conditions. Here, we describe a typical 2DE procedure for plasma proteome under either a healthy or a diseased state (e.g., liver cancer) in which pre-fractionation and depletion are integral steps in the search for disease biomarkers.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1064-3745
pubmed:author
pubmed:issnType
Print
pubmed:volume
428
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
57-75
pubmed:meshHeading
pubmed-meshheading:18287768-Blood Protein Electrophoresis, pubmed-meshheading:18287768-Blood Proteins, pubmed-meshheading:18287768-Carcinoma, Hepatocellular, pubmed-meshheading:18287768-Chromatography, Affinity, pubmed-meshheading:18287768-Chromatography, High Pressure Liquid, pubmed-meshheading:18287768-Electrophoresis, Gel, Two-Dimensional, pubmed-meshheading:18287768-Humans, pubmed-meshheading:18287768-Isoelectric Focusing, pubmed-meshheading:18287768-Liver Neoplasms, pubmed-meshheading:18287768-Peptide Mapping, pubmed-meshheading:18287768-Protein Array Analysis, pubmed-meshheading:18287768-Proteome, pubmed-meshheading:18287768-Proteomics, pubmed-meshheading:18287768-Spectrometry, Mass, Matrix-Assisted Laser..., pubmed-meshheading:18287768-Staining and Labeling, pubmed-meshheading:18287768-Tumor Markers, Biological
pubmed:year
2008
pubmed:articleTitle
Protein profiling of human plasma samples by two-dimensional electrophoresis.
pubmed:affiliation
Department of Biochemistry, College of Sciences, Yonsei Biomedical Proteome Research Center, Seoul, Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't