Source:http://linkedlifedata.com/resource/pubmed/id/18074356
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
2008-1-2
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| pubmed:abstractText |
DNA immunization has been used to induce either humoral or cellular immune responses against many antigens, including hepatitis C virus (HCV). In addition, DNA immunizations can be enhanced or modulated at the nucleotide level. Genetic immunizations were examined in BALB/c mice through the use of plasmids and chimeric DNA constructs encoding HCV core proteins and hepatitis B virus (HBV) precore (preC) regions. Plasmids encoding the truncated HCV core induced potent humoral and cellular responses to HCV; pcDNA3.0A-C154 produced a stronger antibody response than pcDNA3.0A-C191 (P < 0.01) and pcDNA3.0A-C69 (P < 0.05). HBV preC enhanced the humoral and cellular immune responses of BALB/c mice to HCV; however, pcDNA3.0A-C69preC resulted in a weak cytotoxic T lymphocyte (CTL) response. In addition, the humoral and cellular immune responses to HCV of groups immunized with pcDNA3.0A-C154preC and pcDNA3.0A-C191preC plasmids were higher than those of groups immunized with pcDNA3.0A-C154 and pcDNA3.0A-C191. In vivo CTL responses verified that mice immunized with preC core fused DNAs showed significantly high specific lysis compared with mice immunized with HCV cores only (P < 0.01). In our study, pcDNA3.0A-C154preC led to the highest immune response among all DNA constructs. Conclusion: DNA that encodes truncated HCV core proteins may lead to increased immune responses in vivo, and these responses may be enhanced by HBV preC.
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| pubmed:commentsCorrections | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
1527-3350
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| pubmed:author |
pubmed-author:BiShengliS,
pubmed-author:BianTaoT,
pubmed-author:ChangJinhaiJ,
pubmed-author:ChenJunyingJ,
pubmed-author:JiangShudeS,
pubmed-author:LiWeidongW,
pubmed-author:LiaoGuoyangG,
pubmed-author:OmataMasaoM,
pubmed-author:SunMingboM,
pubmed-author:TanWenjieW,
pubmed-author:WangYueY,
pubmed-author:YangHuijuanH,
pubmed-author:ZhangXinwenX
|
| pubmed:issnType |
Electronic
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| pubmed:volume |
47
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
25-34
|
| pubmed:dateRevised |
2008-7-7
|
| pubmed:meshHeading |
pubmed-meshheading:18074356-Animals,
pubmed-meshheading:18074356-Antibody Formation,
pubmed-meshheading:18074356-Cell Proliferation,
pubmed-meshheading:18074356-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:18074356-Female,
pubmed-meshheading:18074356-Gene Expression,
pubmed-meshheading:18074356-Hepacivirus,
pubmed-meshheading:18074356-Hepatitis B virus,
pubmed-meshheading:18074356-Hepatitis C,
pubmed-meshheading:18074356-Immunity, Cellular,
pubmed-meshheading:18074356-Immunization,
pubmed-meshheading:18074356-Mice,
pubmed-meshheading:18074356-Mice, Inbred BALB C,
pubmed-meshheading:18074356-Plasmids,
pubmed-meshheading:18074356-Recombinant Fusion Proteins,
pubmed-meshheading:18074356-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:18074356-Viral Core Proteins,
pubmed-meshheading:18074356-Viral Hepatitis Vaccines
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| pubmed:year |
2008
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| pubmed:articleTitle |
Hepatitis B virus precore protein augments genetic immunizations of the truncated hepatitis C virus core in BALB/c mice.
|
| pubmed:affiliation |
Department of Viral Immunology, Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Yunnan Province, People's Republic of China.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|