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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1992-4-30
|
| pubmed:abstractText |
By indirect immunofluorescence and immunogold electron microscopy with an antibody that recognizes specifically the two forms of native mature rat cathepsin B (31 kDa and 5:25 kDa) but not the proenzyme, we detected cathepsin B not only in lysosomes of adult rat exocrine pancreatic cells but also in the trans Golgi condensing vacuoles, the zymogen granules and the pancreatic juice in the intralobular ducts. In contrast, immunocytochemistry with an antibody specific for rat cathepsin D showed the latter to be present in the same cells only in lysosomal compartments as expected. The same pattern of labeling with these two antibodies was found in the first zymogen granules to form in 17-day-old fetal rat pancreas. Counts of the extent of immunogold labeling of cathepsin B in the adult exocrine cells showed that the concentration of the enzyme was only two-fold higher in the lysosomal compartments than in the zymogen granules. To confirm these observations, rat pancreatic postnuclear supernatant (PNS), a fraction enriched in zymogen granules and rat pancreatic juice obtained by catheterization of the pancreatic duct, were subjected to 2D gel electrophoresis followed by immunoblotting with the cathepsin B antibody. All three samples contained a 31 kDa protein recognized by the antibody with a pI of about 4.5, the single chain mature form of cathepsin B. We then radiolabeled pancreatic PNS and zymogen granule fractions with benzyloxycarbonyl-Tyr[125I]-Ala-CHN2, an affinity label that covalently binds to the active sites of mature forms of both cathepsin B and cathepsin L. In both PNS and zymogen granule fractions this reagent labeled cathepsin B. Immunoprecipitation experiments showed that the antibody to cathepsin B recognized specifically both the single chain and the double chain mature forms of cathepsin B in the native state. Finally, Northern blots with a cDNA of rat cathepsin B showed that the concentration of cathepsin B mRNA in total pancreatic RNA increased following in vivo stimulation of the exocrine pancreatic cells with optimal doses of cerulein, a cholecystokinin analogue. We conclude that significant amounts of mature cathepsin B are secreted from exocrine pancreatic cells via the apical regulated exocytotic pathway, and we discuss this in terms of models for sorting of proteins to the cores of dense cored secretory granules.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Caerulein,
http://linkedlifedata.com/resource/pubmed/chemical/Cathepsin B,
http://linkedlifedata.com/resource/pubmed/chemical/Cathepsin D,
http://linkedlifedata.com/resource/pubmed/chemical/Cathepsin L,
http://linkedlifedata.com/resource/pubmed/chemical/Cathepsins,
http://linkedlifedata.com/resource/pubmed/chemical/Ctsl protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Cysteine Endopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/Dexamethasone,
http://linkedlifedata.com/resource/pubmed/chemical/Endopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Precursors
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0171-9335
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
56
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
187-200
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| pubmed:dateRevised |
2009-11-19
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| pubmed:meshHeading |
pubmed-meshheading:1802707-Aging,
pubmed-meshheading:1802707-Animals,
pubmed-meshheading:1802707-Caerulein,
pubmed-meshheading:1802707-Cathepsin B,
pubmed-meshheading:1802707-Cathepsin D,
pubmed-meshheading:1802707-Cathepsin L,
pubmed-meshheading:1802707-Cathepsins,
pubmed-meshheading:1802707-Cysteine Endopeptidases,
pubmed-meshheading:1802707-Cytoplasmic Granules,
pubmed-meshheading:1802707-Dexamethasone,
pubmed-meshheading:1802707-Embryo, Mammalian,
pubmed-meshheading:1802707-Endopeptidases,
pubmed-meshheading:1802707-Enzyme Precursors,
pubmed-meshheading:1802707-Immunohistochemistry,
pubmed-meshheading:1802707-Lysosomes,
pubmed-meshheading:1802707-Pancreas,
pubmed-meshheading:1802707-Pancreatic Juice,
pubmed-meshheading:1802707-Rats,
pubmed-meshheading:1802707-Rats, Inbred Strains,
pubmed-meshheading:1802707-Subcellular Fractions,
pubmed-meshheading:1802707-Tumor Cells, Cultured,
pubmed-meshheading:1802707-Up-Regulation,
pubmed-meshheading:1802707-Vacuoles
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| pubmed:year |
1991
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| pubmed:articleTitle |
Regulated secretion of mature cathepsin B from rat exocrine pancreatic cells.
|
| pubmed:affiliation |
European Molecular Biology Laboratory, Heidelberg/Federal Republic of Germany.
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| pubmed:publicationType |
Journal Article
|