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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2007-12-28
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pubmed:abstractText |
An experimental DNA plasmid vaccine was developed based on a well-characterized and protective peptide epitope derived from a bacterial porin protein. For this study, we used the P1.16b serosubtype epitope, located in variable region (VR)2 in loop 4 of the PorA outer membrane (OM) porin from Neisseria meningitidis serogroup B strain MC58. A plasmid that encoded the entire loop (pPorAloop4) was prepared, as well as a fusion plasmid that encoded the loop in tandem with the fragment C (FrC) immunostimulatory sequence from tetanus toxin (pPorAloop4-FrC). The constructs were used for intramuscular immunization without exogenous adjuvant. Murine antisera raised to the pPorAloop4-FrC DNA fusion plasmid reacted significantly with OMs in enzyme-linked immunosorbent assay and with whole bacteria by immunofluorescence, whereas antisera raised to the pPorAloop4 DNA plasmid and to control plasmid showed little or no reactivity. Significantly, only the pPorALoop4-FrC plasmid induced bactericidal antibodies, demonstrating that the intrinsic immunostimulatory sequence was essential for inducing a protective immune response. The antibodies raised to the P1.16b pPorALoop4-FrC plasmid were serosubtype specific, showing no significant immunofluorescence reactivity or bactericidal activity against other PorA variants. These data provide proof of principle for a DNA fusion plasmid strategy as a novel approach to preparing vaccines based on defined, protective epitopes.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17967859-10540159,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17967859-10669329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17967859-10699338,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/17967859-11282194,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17967859-11292786,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17967859-11349046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17967859-11401993,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/17967859-14975255,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17967859-15501781,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/17967859-9846738
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1098-5522
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
76
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
334-8
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pubmed:dateRevised |
2011-4-7
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pubmed:meshHeading |
pubmed-meshheading:17967859-Animals,
pubmed-meshheading:17967859-Antibodies, Bacterial,
pubmed-meshheading:17967859-Bacterial Vaccines,
pubmed-meshheading:17967859-Epitopes,
pubmed-meshheading:17967859-Meningococcal Infections,
pubmed-meshheading:17967859-Mice,
pubmed-meshheading:17967859-Mice, Inbred BALB C,
pubmed-meshheading:17967859-Mice, Inbred C57BL,
pubmed-meshheading:17967859-Neisseria meningitidis,
pubmed-meshheading:17967859-Plasmids,
pubmed-meshheading:17967859-Porins,
pubmed-meshheading:17967859-Vaccines, DNA
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pubmed:year |
2008
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pubmed:articleTitle |
A DNA fusion vaccine induces bactericidal antibodies to a peptide epitope from the PorA porin of Neisseria meningitidis.
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pubmed:affiliation |
Genetic Vaccine Group, Cancer Sciences Division, University of Southampton Medical School, Southampton General Hospital, Southampton SO16 6YD, United Kingdom.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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