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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1992-3-3
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pubmed:abstractText |
The transformed and tumorigenic phenotype of H-ras transfected rat FE-8 cells can be suppressed by cell fusion with normal rat embryo fibroblasts. Transfection into FE-8 cells of DNA prepared from normal human placenta followed by selective elimination of tumorigenic transfected cell clones resulted in the isolation of phenotypically normal revertants. These cells exhibited a fibroblastlike, normal morphology; were anchorage-dependent; and were unable to proliferate in medium with reduced serum concentrations. Their tumorigenicity was also reduced. The suppressed phenotype has been transferred in a second transfection cycle. Human repetitive DNA sequences were detected in secondary transfectant DNA. A putative human suppressor gene, designated NTS-1, has been molecularly cloned. Reintroduction of cloned NTS-1 sequences into FE-8 cells resulted in suppression of the neoplastic phenotype in spite of a high ras expression.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0091-6765
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
93
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pubmed:geneSymbol |
EJ-ras,
H-ras,
K-rasv-src,
NTS-1,
ras,
v-fgr
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
79-82
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:1773805-Animals,
pubmed-meshheading:1773805-Cell Fusion,
pubmed-meshheading:1773805-Cell Line, Transformed,
pubmed-meshheading:1773805-Cell Transformation, Neoplastic,
pubmed-meshheading:1773805-Cricetinae,
pubmed-meshheading:1773805-Cricetulus,
pubmed-meshheading:1773805-Fibroblasts,
pubmed-meshheading:1773805-Genes, Tumor Suppressor,
pubmed-meshheading:1773805-Genes, ras,
pubmed-meshheading:1773805-Humans,
pubmed-meshheading:1773805-Hybrid Cells,
pubmed-meshheading:1773805-Neoplasms, Experimental,
pubmed-meshheading:1773805-Oncogene Proteins, Viral,
pubmed-meshheading:1773805-Oncogenes,
pubmed-meshheading:1773805-Open Reading Frames,
pubmed-meshheading:1773805-Phenotype,
pubmed-meshheading:1773805-Placenta,
pubmed-meshheading:1773805-Protein-Tyrosine Kinases,
pubmed-meshheading:1773805-Rats,
pubmed-meshheading:1773805-Recombinant Proteins,
pubmed-meshheading:1773805-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:1773805-Transfection
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pubmed:year |
1991
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pubmed:articleTitle |
Molecular and functional analysis of tumor-suppressor genes by transfection.
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pubmed:affiliation |
Division of Cancer Research, University of Zürich, Switzerland.
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pubmed:publicationType |
Journal Article,
Review,
Research Support, Non-U.S. Gov't
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