Source:http://linkedlifedata.com/resource/pubmed/id/17616938
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
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| pubmed:dateCreated |
2007-7-9
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| pubmed:abstractText |
The clinical behaviour of Clear Cell Renal Cell Carcinoma (CC-RCC) is often unpredictable. To fully understand the signaling pathways involved in CC-RCC development, we examined whether the 5-Lipoxygenase (5-LO), which catalyzes the biosynthesis of proinflammatory leukotrienes, is involved in renal tumorigenesis. By analyzing 46 snap-frozen primary renal cell carcinomas and their corresponding normal renal cortex biopsies, 5-LO protein levels were found to be significantly increased in the majority of CC-RCCs (P<0.001). Quantitative 5-LO mRNA expression analysis revealed up to 3-fold increased expression in the tumor tissues. There was no association between 5-LO and gender, grade or vein invasion. In contrast, increased 5-LO protein and mRNA correlated with large tumor size (>4 cm) and age of patients (P<0.001). 5-LO was frequently overexpressed in von Hippel-Lindau protein (pVHL)-reduced tumors and in Vascular Endothelial Growth Factor (VEGF)-positive tumors, which represent two frequent alterations in CC-RCC. Cell culture experiments demonstrated that VEGF expression was strongly inducible by 5-LO metabolites in RCC cell lines. The loss of pVHL expression led to high basal 5-LO and VEGF expression, which were markedly reduced by transfection with 5-LO small interfering RNA (siRNA). These results suggest that 5-LO up-regulation is an important step in renal cancer progression.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Arachidonate 5-Lipoxygenase,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Markers, Biological,
http://linkedlifedata.com/resource/pubmed/chemical/VBP1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/VEGFA protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factor A
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| pubmed:status |
MEDLINE
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| pubmed:month |
Oct
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| pubmed:issn |
1699-5848
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
22
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1109-18
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| pubmed:meshHeading |
pubmed-meshheading:17616938-Adenocarcinoma, Clear Cell,
pubmed-meshheading:17616938-Adult,
pubmed-meshheading:17616938-Aged,
pubmed-meshheading:17616938-Aged, 80 and over,
pubmed-meshheading:17616938-Arachidonate 5-Lipoxygenase,
pubmed-meshheading:17616938-Base Sequence,
pubmed-meshheading:17616938-Carcinoma, Renal Cell,
pubmed-meshheading:17616938-Carrier Proteins,
pubmed-meshheading:17616938-Cell Line, Tumor,
pubmed-meshheading:17616938-Female,
pubmed-meshheading:17616938-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:17616938-Humans,
pubmed-meshheading:17616938-Kidney Neoplasms,
pubmed-meshheading:17616938-Male,
pubmed-meshheading:17616938-Middle Aged,
pubmed-meshheading:17616938-Molecular Sequence Data,
pubmed-meshheading:17616938-RNA, Messenger,
pubmed-meshheading:17616938-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:17616938-Tumor Markers, Biological,
pubmed-meshheading:17616938-Up-Regulation,
pubmed-meshheading:17616938-Vascular Endothelial Growth Factor A
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| pubmed:year |
2007
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| pubmed:articleTitle |
Increased expression of 5-lipoxygenase is common in clear cell renal cell carcinoma.
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| pubmed:affiliation |
Department of Molecular Pathology and Innovative Therapies, Polytechnic University of Marche, Ancona, Italy.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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