Linked Life Data

17440494

Source:http://linkedlifedata.com/resource/pubmed/id/17440494

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2007-7-26
pubmed:abstractText
Many clinical and animal studies suggest that vitamin D and its metabolites have beneficial effects in the cardiovascular and renal systems. Using immunologic and enzymatic assays, vitamin D receptor and 25 hydroxyvitamin D3 1alpha-hydroxylase activity were found in inner medullary collecting duct (IMCD) cells suggesting an autocrine/paracrine role in this nephron segment. In this study, we examined the ability of 1,25 dihydroxyvitamin D3 (1,25(OH)(2)D3) to regulate the expression of the vasculoprotective natriuretic peptide receptor-A gene in these cells in culture. Treatment of the cells with 1,25(OH)(2)D3 caused a doubling of natriuretic peptide-dependent cyclic guanosine monophosphate production and a significant increase in natriuretic peptide receptor-A protein expression. This was accompanied by significant increases in receptor mRNA levels and gene-promoter activity. Mutation of a vitamin D response element, positioned upstream from the gene start site, resulted in a complete loss of 1,25(OH)(2)D3-dependent induction but not the induction by hypertonic stimuli. Introduction of small interfering RNA directed against the vitamin D receptor into the IMCD cells resulted in decreased natriuretic peptide receptor-A gene promoter activity and protein. The increase in this receptor expression may account for some of the reported beneficial effect of 1,25(OH)(2)D3 on the cardiovascular system and kidney.
pubmed:grant
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0085-2538
pubmed:author
pubmed:issnType
Print
pubmed:volume
72
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
300-6
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:17440494-Animals, pubmed-meshheading:17440494-Atrial Natriuretic Factor, pubmed-meshheading:17440494-Calcitriol, pubmed-meshheading:17440494-Calcium Channel Agonists, pubmed-meshheading:17440494-Cardiovascular Physiological Phenomena, pubmed-meshheading:17440494-Cells, Cultured, pubmed-meshheading:17440494-Cyclic GMP, pubmed-meshheading:17440494-Guanylate Cyclase, pubmed-meshheading:17440494-Kidney Tubules, Collecting, pubmed-meshheading:17440494-RNA, Messenger, pubmed-meshheading:17440494-RNA, Small Interfering, pubmed-meshheading:17440494-Rats, pubmed-meshheading:17440494-Rats, Sprague-Dawley, pubmed-meshheading:17440494-Receptors, Atrial Natriuretic Factor, pubmed-meshheading:17440494-Sodium, pubmed-meshheading:17440494-Transcription, Genetic, pubmed-meshheading:17440494-Vitamin D
pubmed:year
2007
pubmed:articleTitle
Vitamin D activates type A natriuretic peptide receptor gene transcription in inner medullary collecting duct cells.
pubmed:affiliation
Diabetes Center, University of California at San Francisco, San Francisco, California 94143-0540, USA. schen@diabetes.ucsf.edu
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural