Linked Life Data

17395197

Source:http://linkedlifedata.com/resource/pubmed/id/17395197

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2007-5-8
pubmed:abstractText
The objective of this study was to investigate the presence of Thy-1 in the myocardium and on cardiac fibroblasts and to determine whether or not cardiac fibroblasts form a heterogeneous population in term of Thy-1 expression. Thy-1 expression was examined by immunohistology of ventricular sections from normal and deoxycorticosterone acetate (DOCA)-salt hypertensive rats. Thy-1 immunostaining was detected in connective tissue on alpha8 integrin-positive and discoidin domain receptor 2 (DDR2)-positive fibroblasts. Enhanced Thy-1 staining was observed in the hearts of DOCA-salt rats particularly in areas of interstitial fibrosis. Cardiac mRNA analysis confirmed the increased Thy-1 expression. On cultured cardiac fibroblasts, flow cytofluorometry showed that cells, from primary culture to passage 4, were double positive for Thy-1 and for both alpha8 integrin and DDR2. Analysis of isolated lipid rafts by detergent-free sucrose gradient indicated that Thy-1 protein was probably located in these structures, but it may be located on a membrane microdomain slightly different from those of caveolin-1, as revealed by immunocytochemistry. Differentiation of fibroblasts into myofibroblasts being a characteristic of cardiac fibrosis and scarring, cardiac fibroblasts were stimulated in the presence of transforming growth factor-beta (TGF-beta) or connective tissue growth factor. While the expression of smooth muscle alpha-actin and alpha8 integrin doubled, Thy-1 level, measured by Western blotting and flow cytofluorometry, was not influenced by TGF-beta. These results demonstrate that cardiac fibroblasts express Thy-1 and form a homogeneous population. Thy-1 expression also appears to be independent of fibroblast differentiation. The dichotomy between the increased Tthy-1 expression in the fibrotic area and the lack of association with fibroblast differentiation suggests that Thy-1 may represent a marker of fibroblast proliferation in the myocardium.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0022-2828
pubmed:author
pubmed:issnType
Print
pubmed:volume
42
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
991-1000
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Thy-1 expression by cardiac fibroblasts: lack of association with myofibroblast contractile markers.
pubmed:affiliation
Institut de recherches cliniques de Montréal, Université de Montréal, 110, Avenue des Pins ouest, Montréal, Québec, Canada H2W 1R7.
pubmed:publicationType
Journal Article