Linked Life Data

17265860

Source:http://linkedlifedata.com/resource/pubmed/id/17265860

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2007-2-1
pubmed:abstractText
Since the outbreak of foodborne illness linked to Escherichia coli O157:H7 bacteria in ground beef in the early 1980s, the beef processing industry has focused on increasing the safety of beef products by implementing procedures for surveying live cattle, carcasses, and beef products for bacterial pathogens. Effective methods are in place for screening cattle and beef products for the presence of E. coli O157:H7 contamination, and recent work has established the acceptability of these methods for surveillance of Salmonella. In keeping with the need to continually improve the food safety of beef products, new work investigating pathogen prevalence now includes surveillance for Listeria monocytogenes. Tryptic soy broth (TSB) has been documented as a robust nonselective medium for the enrichment of both E. coli and Salmonella from bovine hide, carcass, and meat samples. The University of Vermont modification medium is most often used as the primary enrichment medium for surveillance of Listeria spp. In this study, samples from bovine hides (n=50), preevisceration carcasses (n=50), and beef trim (n=193) were used to evaluate TSB as a primary enrichment medium for the isolation of Listeria spp., including L. monocytogenes. No significant difference (P > 0.05) between TSB and the University of Vermont modification medium was observed when all three sample types underwent primary enrichment for the isolation of Listeria spp. Furthermore, the standard secondary enrichment ratio for Fraser broth used for Listeria recovery can be modified to accommodate a high-throughput method for processing multiple samples.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0362-028X
pubmed:author
pubmed:issnType
Print
pubmed:volume
70
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
53-7
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Rapid enrichment strategy for isolation of Listeria from bovine hide, carcass, and meat samples.
pubmed:affiliation
U.S. Department of Agriculture, Agricultural Research Service, Roman L. Hruska U.S. Meat Animal Research Center, Spur 18-D, Clay Center, Nebraska 68933-0166, USA. guerini@email.marc.usda.gov
pubmed:publicationType
Journal Article