Source:http://linkedlifedata.com/resource/pubmed/id/17256962
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
2007-2-15
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pubmed:abstractText |
Betalains are an important class of water-soluble pigments, with radical scavenging capacity, which is characteristic of the order Caryophyllales. The structural unit of the violet betacyanins, betanidin is reported as a substrate for the enzyme tyrosinase (EC 1.14.18.1), which plays a key role in the betalains biosynthetic scheme. The compound was identified in Lampranthus productus violet flowers, from which it was extracted and purified. The tyrosinase-mediated oxidation of betanidin was characterized in depth and followed by high-performance liquid chromatography and spectrophotometry. The addition of ascorbic acid reversed the reaction product, betanidin-quinone, to the original pigment. Kinetic analysis revealed a Km = 0.66 mM. Betanidin degradation kinetics was also studied in the absence of the enzyme and demonstrated that pH values over 6.0 and high ionic strength reduce the pigment stability.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0021-8561
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
21
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pubmed:volume |
55
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1546-51
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pubmed:meshHeading |
pubmed-meshheading:17256962-Angiosperms,
pubmed-meshheading:17256962-Ascorbic Acid,
pubmed-meshheading:17256962-Betacyanins,
pubmed-meshheading:17256962-Chromatography, High Pressure Liquid,
pubmed-meshheading:17256962-Flowers,
pubmed-meshheading:17256962-Kinetics,
pubmed-meshheading:17256962-Monophenol Monooxygenase,
pubmed-meshheading:17256962-Oxidation-Reduction,
pubmed-meshheading:17256962-Spectrophotometry
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pubmed:year |
2007
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pubmed:articleTitle |
Characterization of the activity of tyrosinase on betanidin.
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pubmed:affiliation |
Departamento de Bioquímica y Biología Molecular A, Unidad Docente de Biología, Facultad de Veterinaria, Universidad de Murcia, E-30100 Espinardo, Murcia, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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