Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2007-2-15
pubmed:abstractText
Betalains are an important class of water-soluble pigments, with radical scavenging capacity, which is characteristic of the order Caryophyllales. The structural unit of the violet betacyanins, betanidin is reported as a substrate for the enzyme tyrosinase (EC 1.14.18.1), which plays a key role in the betalains biosynthetic scheme. The compound was identified in Lampranthus productus violet flowers, from which it was extracted and purified. The tyrosinase-mediated oxidation of betanidin was characterized in depth and followed by high-performance liquid chromatography and spectrophotometry. The addition of ascorbic acid reversed the reaction product, betanidin-quinone, to the original pigment. Kinetic analysis revealed a Km = 0.66 mM. Betanidin degradation kinetics was also studied in the absence of the enzyme and demonstrated that pH values over 6.0 and high ionic strength reduce the pigment stability.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0021-8561
pubmed:author
pubmed:issnType
Print
pubmed:day
21
pubmed:volume
55
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1546-51
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Characterization of the activity of tyrosinase on betanidin.
pubmed:affiliation
Departamento de Bioquímica y Biología Molecular A, Unidad Docente de Biología, Facultad de Veterinaria, Universidad de Murcia, E-30100 Espinardo, Murcia, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't