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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1990-5-2
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pubmed:abstractText |
cAMP-dependent protein kinase (PKA) and phospholipid-dependent protein kinase (PKC) play a role in nerve growth factor (NGF)-mediated differentiation. In PC12 cells, NGF causes neurite outgrowth and increases the number of voltage-gated Na+ channels. Neurite outgrowth involves in part activation of PKC. How NGF regulates Na+ channel number is unknown. Using patch-clamp techniques, we find that agents activating PKC, including phorbol esters and a ras oncogene product (p21) that induces neurites, caused little increase in channel number. In contrast, agents increasing intracellular cAMP were as effective as NGF. A specific protein inhibitor of the PKA catalytic subunit blocked increases by NGF or cAMP. Thus, NGF increases Na+ channel number in PC12 cells in part by activating PKA but apparently not PKC.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1-Methyl-3-isobutylxanthine,
http://linkedlifedata.com/resource/pubmed/chemical/Bucladesine,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic CMP,
http://linkedlifedata.com/resource/pubmed/chemical/Dibutyryl Cyclic GMP,
http://linkedlifedata.com/resource/pubmed/chemical/Dimethyl Sulfoxide,
http://linkedlifedata.com/resource/pubmed/chemical/Forskolin,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Tetrodotoxin,
http://linkedlifedata.com/resource/pubmed/chemical/dibutyryl cyclic-3',5'-cytidine...
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0896-6273
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
4
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
355-66
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:1690563-1-Methyl-3-isobutylxanthine,
pubmed-meshheading:1690563-Adrenal Gland Neoplasms,
pubmed-meshheading:1690563-Animals,
pubmed-meshheading:1690563-Bucladesine,
pubmed-meshheading:1690563-Cell Differentiation,
pubmed-meshheading:1690563-Cell Line,
pubmed-meshheading:1690563-Cyclic CMP,
pubmed-meshheading:1690563-Dibutyryl Cyclic GMP,
pubmed-meshheading:1690563-Dimethyl Sulfoxide,
pubmed-meshheading:1690563-Electric Conductivity,
pubmed-meshheading:1690563-Electrophysiology,
pubmed-meshheading:1690563-Forskolin,
pubmed-meshheading:1690563-Gene Expression,
pubmed-meshheading:1690563-Genes, ras,
pubmed-meshheading:1690563-Kinetics,
pubmed-meshheading:1690563-Nerve Growth Factors,
pubmed-meshheading:1690563-Pheochromocytoma,
pubmed-meshheading:1690563-Protein Kinases,
pubmed-meshheading:1690563-Rats,
pubmed-meshheading:1690563-Sodium Channels,
pubmed-meshheading:1690563-Tetrodotoxin
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pubmed:year |
1990
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pubmed:articleTitle |
Nerve growth factor acts through cAMP-dependent protein kinase to increase the number of sodium channels in PC12 cells.
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pubmed:affiliation |
Department of Biology, University of California, Los Angeles 90024.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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