Linked Life Data

16809629

Source:http://linkedlifedata.com/resource/pubmed/id/16809629

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2006-10-17
pubmed:abstractText
The primary purpose of this study was to determine the effect of prior exercise on insulin-stimulated glucose uptake with physiological insulin in isolated muscles of mice. Male C57BL/6 mice completed a 60-min treadmill exercise protocol or were sedentary. Paired epitrochlearis, soleus, and extensor digitorum longus (EDL) muscles were incubated with [3H]-2-deoxyglucose without or with insulin (60 microU/ml) to measure glucose uptake. Insulin-stimulated glucose uptake for paired muscles was calculated by subtracting glucose uptake without insulin from glucose uptake with insulin. Muscles from other mice were assessed for glycogen and AMPK Thr172 phosphorylation. Exercised vs. sedentary mice had decreased glycogen in epitrochlearis (48%, P < 0.001), soleus (51%, P < 0.001), and EDL (41%, P < 0.01) and increased AMPK Thr172 phosphorylation (P < 0.05) in epitrochlearis (1.7-fold), soleus (2.0-fold), and EDL (1.4-fold). Insulin-independent glucose uptake was increased 30 min postexercise vs. sedentary in the epitrochlearis (1.2-fold, P < 0.001), soleus (1.4-fold, P < 0.05), and EDL (1.3-fold, P < 0.01). Insulin-stimulated glucose uptake was increased (P < 0.05) approximately 85 min after exercise in the epitrochlearis (sedentary: 0.266 +/- 0.045 micromol x g(-1) x 15 min(-1); exercised: 0.414 +/- 0.051) and soleus (sedentary: 0.102 +/- 0.049; exercised: 0.347 +/- 0.098) but not in the EDL. Akt Ser473 and Akt Thr308 phosphorylation for insulin-stimulated muscles did not differ in exercised vs. sedentary. These results demonstrate enhanced submaximal insulin-stimulated glucose uptake in the epitrochlearis and soleus of mice 85 min postexercise and suggest that it will be feasible to probe the mechanism of enhanced postexercise insulin sensitivity by using genetically modified mice.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
8750-7587
pubmed:author
pubmed:issnType
Print
pubmed:volume
101
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1368-76
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:16809629-AMP-Activated Protein Kinases, pubmed-meshheading:16809629-Animals, pubmed-meshheading:16809629-Deoxyglucose, pubmed-meshheading:16809629-Dose-Response Relationship, Drug, pubmed-meshheading:16809629-Exercise Test, pubmed-meshheading:16809629-Glucose, pubmed-meshheading:16809629-Glycogen, pubmed-meshheading:16809629-Hypoglycemic Agents, pubmed-meshheading:16809629-Insulin, pubmed-meshheading:16809629-Male, pubmed-meshheading:16809629-Mice, pubmed-meshheading:16809629-Mice, Inbred C57BL, pubmed-meshheading:16809629-Multienzyme Complexes, pubmed-meshheading:16809629-Muscle, Skeletal, pubmed-meshheading:16809629-Phosphorylation, pubmed-meshheading:16809629-Physical Conditioning, Animal, pubmed-meshheading:16809629-Protein-Serine-Threonine Kinases, pubmed-meshheading:16809629-Serine, pubmed-meshheading:16809629-Threonine, pubmed-meshheading:16809629-Tritium
pubmed:year
2006
pubmed:articleTitle
Increased submaximal insulin-stimulated glucose uptake in mouse skeletal muscle after treadmill exercise.
pubmed:affiliation
Muscle Biology Laboratory, Division of Kinesiology, University of Michigan, Ann Arbor, MI 48109-2214, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural