Linked Life Data

1675512

Source:http://linkedlifedata.com/resource/pubmed/id/1675512

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1991-7-18
pubmed:abstractText
We have examined the histological and cytoskeletal changes in rat connective tissues induced by subcutaneous perfusion with cytokines. Granulocyte macrophage-colony stimulating factor (GM-CSF), tumor necrosis factor-alpha (TNF-alpha), interleukin-1-alpha (IL-1-alpha), transforming growth factor-beta (TGF-beta) and platelet-derived growth factor (PDGF) produced a significant fibroblast accumulation, neovascular development and a weak to moderate leukocyte infiltration, while interleukin-2 (IL-2) and gamma-interferon (gamma-IFN) induced intense mononucleated leukocyte infiltration. Immunofluorescence staining showed that accumulated fibroblastic cells were positive for alpha-smooth muscle (SM) actin (but negative for the desmin and muscle myosin) only in GM-CSF-treated tissues. Electron microscopic examination established that a significant proportion of fibroblastic cell in GM-CSF-, IL-1-alpha- or TGF-beta-treated animals were typical myofibroblasts. Only in GM-CSF-treated animals did microfilament bundles of myofibroblasts contain alpha-SM actin, when examined by immuno electron microscopy. Our results suggest that locally applied cytokines induce the formation of distinct granulation tissues. In particular, GM-CSF stimulates alpha-SM actin synthesis in myofibroblasts, illustrating an unexpected extra-hematopoietic in vivo effect of this factor.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0340-6075
pubmed:author
pubmed:issnType
Print
pubmed:volume
60
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
73-82
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:1675512-Actins, pubmed-meshheading:1675512-Animals, pubmed-meshheading:1675512-Female, pubmed-meshheading:1675512-Fibroblasts, pubmed-meshheading:1675512-Fluorescent Antibody Technique, pubmed-meshheading:1675512-Granulation Tissue, pubmed-meshheading:1675512-Granulocyte-Macrophage Colony-Stimulating Factor, pubmed-meshheading:1675512-Interleukin-1, pubmed-meshheading:1675512-Leukocytes, pubmed-meshheading:1675512-Microscopy, Immunoelectron, pubmed-meshheading:1675512-Muscle, Smooth, pubmed-meshheading:1675512-Platelet-Derived Growth Factor, pubmed-meshheading:1675512-Rats, pubmed-meshheading:1675512-Rats, Inbred Strains, pubmed-meshheading:1675512-Transforming Growth Factor beta, pubmed-meshheading:1675512-Tumor Necrosis Factor-alpha, pubmed-meshheading:1675512-Wound Healing
pubmed:year
1991
pubmed:articleTitle
Locally applied GM-CSF induces the accumulation of alpha-smooth muscle actin containing myofibroblasts.
pubmed:affiliation
Department of Pathology, University of Geneva, Switzerland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't