Linked Life Data

1666828

Source:http://linkedlifedata.com/resource/pubmed/id/1666828

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1992-5-6
pubmed:abstractText
Three types of antibody, which were specific to the purified human protein C inhibitor (PCI) and to two synthetic peptides, which corresponded to the N-terminal 15 amino acid residues (PCI-N) and C-terminal 15 residues (PCI-C) of PCI, were produced. Plasma PCI patterns, which were shown by means of polyacrylamide gel isoelectric focusing followed by immunoblotting with the antibody to purified PCI, were almost identical to those obtained with the antibodies to the peptides, PCI-N and PCI-C. The latter antibodies against the synthetic peptides proved very useful for biochemical or genetic analysis of human plasma PCI, as such peptide antigens could be produced without any of the tedious procedures necessary to obtain purified PCI as an immunogen.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0158-5231
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
553-60
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Novel detection method for human protein C inhibitor (PCI) using immunoblotting with antibodies specific to synthetic peptides corresponding to the N-terminal and C-terminal amino acid sequences of human PCI.
pubmed:affiliation
Department of Legal Medicine, Fukui Medical School, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't