Linked Life Data

16626785

Source:http://linkedlifedata.com/resource/pubmed/id/16626785

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2-3
pubmed:dateCreated
2006-7-31
pubmed:abstractText
A study on the assimilation and detoxification of selenium and mercury and on the interaction between these two elements was conducted on Pseudomonas fluorescens. P. fluorescens was able to convert separately both elements to their elemental forms, which are less toxic and biologically less available. To study the converting mechanism of selenite to elemental Se, cells were grown in the presence of various selenite concentrations and several parameters such as extracellular protein concentrations, pH, carbohydrate concentrations, isocitrate dehydrogenase (ICDH) and malic enzyme were monitored. Transmission electron microscopy (TEM) and various analytical methods were applied to confirm the interaction between selenium and cell. The former appeared as a red precipitate localized predominantly in the consumed culture medium. P. fluorescens also resisted to the toxic effect of mercury by converting Hg2+ to the volatile and less toxic form Hg0. Mercury reductase was likely responsible for the conversion of Hg2+ to Hg0. More importantly, the interaction between mercury and selenium was also studied. The presence of selenite significantly reduced the accumulation of mercury in P. fluorescens. It was also interesting to note that mercury appeared to behave as a protecting agent against selenium intoxication as the bioaccumulation of Se was also inhibited by this metal. The formation of Se-Hg complexes could explain this mutual protective effect. No precipitate of elemental Se could be detected when Hg was present in the cultures.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0048-9697
pubmed:author
pubmed:issnType
Print
pubmed:day
31
pubmed:volume
367
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
704-14
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Detoxification of selenite and mercury by reduction and mutual protection in the assimilation of both elements by Pseudomonas fluorescens.
pubmed:affiliation
Department of Chemistry and Biochemistry, Laurentian University, Sudbury, Ontario, Canada, P3E 2C6. nbelzile@laurentian.ca
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't