Linked Life Data

16583402

Source:http://linkedlifedata.com/resource/pubmed/id/16583402

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2006-5-16
pubmed:abstractText
The prevailing view is that the glutamine (Gln) transporter (GlnT/ATA1/SAT1/SNAT1) is a member of the system A transporter superfamily with the ability to fuel the glutamate/Gln cycle at nerve terminals in glutamatergic neurons. Semiquantitative reverse transcription-polymerase chain reaction revealed similarly high expression of mRNA for GlnT by rat brain neocortical astrocytes as well as neurons, with progressively lower expression by cerebellar astrocytes, hippocampal astrocytes, and whole-brain microglia in culture. [(3)H]Gln was accumulated in a temperature-dependent manner with a saturable profile in both cultured neocortical neurons and astrocytes, whereas biochemical and pharmacological analyses on [(3)H]Gln accumulation revealed the expression of both system A and system L transporters by cultured neocortical neurons and astrocytes. Exposure to lipopolysaccharide (LPS) for 24 hr resulted in a significant decrease in both GlnT mRNA expression and [(3)H]Gln accumulation, with a concomitant drastic increase in nitrite formation in cultured neocortical astrocytes. Moreover, LPS significantly inhibited the promoter activity of GlnT in the astrocytic cell line C6 glioma cells as well as primary rat neocortical astrocytes in culture. These results suggest that activation by LPS would lead to down-regulation of the expression of GlnT responsible for the incorporation of extracellular Gln into intracellular spaces across plasma membranes through the inhibition of its promoter activity in cultured rat neocortical astrocytes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0360-4012
pubmed:author
pubmed:copyrightInfo
(c) 2006 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:volume
83
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1447-60
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:16583402-Amino Acid Transport System A, pubmed-meshheading:16583402-Animals, pubmed-meshheading:16583402-Animals, Newborn, pubmed-meshheading:16583402-Astrocytes, pubmed-meshheading:16583402-Base Sequence, pubmed-meshheading:16583402-Carrier Proteins, pubmed-meshheading:16583402-Cell Line, Tumor, pubmed-meshheading:16583402-Cell Membrane, pubmed-meshheading:16583402-Cells, Cultured, pubmed-meshheading:16583402-Down-Regulation, pubmed-meshheading:16583402-Encephalitis, pubmed-meshheading:16583402-Endocytosis, pubmed-meshheading:16583402-Glutamic Acid, pubmed-meshheading:16583402-Lipopolysaccharides, pubmed-meshheading:16583402-Molecular Sequence Data, pubmed-meshheading:16583402-Neocortex, pubmed-meshheading:16583402-Promoter Regions, Genetic, pubmed-meshheading:16583402-RNA, Messenger, pubmed-meshheading:16583402-Rats, pubmed-meshheading:16583402-Rats, Wistar
pubmed:year
2006
pubmed:articleTitle
Functional expression of A glutamine transporter responsive to down-regulation by lipopolysaccharide through reduced promoter activity in cultured rat neocortical astrocytes.
pubmed:affiliation
Laboratory of Molecular Pharmacology, Division of Pharmaceutical Sciences, Kanazawa University Graduate School of Natural Science and Technology, Kanazawa, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't