Source:http://linkedlifedata.com/resource/pubmed/id/16491604
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
2006-2-22
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| pubmed:abstractText |
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting to detect proteins and glycoproteins is one of the most widely used and broadly useful techniques in cancer research, allowing the proteins in a complex sample--such as a blood sample, aspirate, or solid tumor homogenate--to be separated according to molecular weight and visualized within a gel matrix and/or, once separated, transferred onto a supporting membrane, where they may be probed for the binding of antibodies or lectins. In this chapter, the theory and principles of SDS-PAGE and Western blotting are briefly outlined, and basic methods are given that can be applied to investigate virtually any (glyco)protein of interest in breast cancer research.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:issn |
1543-1894
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
120
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
217-29
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| pubmed:meshHeading |
pubmed-meshheading:16491604-Blotting, Western,
pubmed-meshheading:16491604-Breast Neoplasms,
pubmed-meshheading:16491604-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:16491604-Female,
pubmed-meshheading:16491604-Glycoproteins,
pubmed-meshheading:16491604-Humans,
pubmed-meshheading:16491604-Neoplasm Proteins,
pubmed-meshheading:16491604-Tumor Markers, Biological
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| pubmed:year |
2006
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| pubmed:articleTitle |
SDS-PAGE and Western blotting to detect proteins and glycoproteins of interest in breast cancer research.
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| pubmed:affiliation |
Department of Physiology, University of Birmingham Medical School, UK.
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| pubmed:publicationType |
Journal Article
|