SDS-PAGE

Source:http://linkedlifedata.com/resource/umls/id/C0600209

CSP: electrophoretic technique for estimating molecular weights of polypeptides by reducing all disulfide bonds, saturating with the anionic detergent sodium dodecylsulfate (SDS), and electrophoresing on polyacrylamide gels.,NCI: Sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis is a procedure for estimating molecular weights of polypeptides, and uses SDS, a detergent that dissociates and unfolds oligomeric proteins into its subunits. The SDS binds to the polypeptides to form complexes with fairly constant charge to mass ratios. The electrophoretic migration rate through a gel is therefore determined only by the size of the complexes.,MSH: Polyacrylamide gel electrophoresis using SODIUM DODECYL SULFATE to denature proteins and separate them on the basis of their molecular weights.

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