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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
44
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pubmed:dateCreated |
2005-11-2
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pubmed:abstractText |
MicroRNAs (miRNAs) regulate posttranscriptional gene activity by binding to specific sequences in the 3' UTRs of target mRNAs. A number of metazoan miRNAs have been shown to exhibit tissue-specific patterns of expression. Here, we investigate the possibility that localized expression is mediated by tissue-specific enhancers, comparable to those seen for protein-coding genes. Two miRNA loci in Drosophila melanogaster are investigated, the mir-309-6 polycistron (8-miR) and the mir-1 gene. The 8-miR locus contains a cluster of eight distinct miRNAs that are transcribed in a common precursor RNA. The 8-miR primary transcript displays a dynamic pattern of expression in early embryos, including repression at the anterior and posterior poles. An 800-bp 5' enhancer was identified that recapitulates this complex pattern when attached to a RNA polymerase II core promoter fused to a lacZ-reporter gene. The miR-1 locus is specifically expressed in the mesoderm of gastrulating embryos. Bioinformatics methods were used to identify a mesoderm-specific enhancer located approximately 5 kb 5' of the miR-1 transcription unit. Evidence is presented that the 8-miR enhancer is regulated by the localized Huckebein repressor, whereas miR-1 is activated by Dorsal and Twist. These results provide evidence that restricted activities of the 8-miR and miR-1 miRNAs are mediated by classical tissue-specific enhancers.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16249329-11597829,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16249329-11752406,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16249329-12198168,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16249329-12464180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16249329-12871707,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16249329-14654021,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16249329-15075295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16249329-15143321,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16249329-15203022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16249329-15297669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16249329-15361871,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16249329-15372042,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16249329-15372072,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16249329-15380237,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16249329-15525708,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16249329-15610845,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16249329-15723116,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16249329-15797203,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16249329-15852042,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16249329-15951802,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16249329-16122423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16249329-1655572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16249329-2176634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16249329-3412488,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16249329-6289436
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Drosophila Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Hkb protein, Drosophila,
http://linkedlifedata.com/resource/pubmed/chemical/MicroRNAs,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA Precursors,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Twi protein, Drosophila,
http://linkedlifedata.com/resource/pubmed/chemical/Twist Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/dl (dorsal) protein, Drosophila
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
102
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
15907-11
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:16249329-Animals,
pubmed-meshheading:16249329-DNA-Binding Proteins,
pubmed-meshheading:16249329-Drosophila Proteins,
pubmed-meshheading:16249329-Drosophila melanogaster,
pubmed-meshheading:16249329-Embryo, Nonmammalian,
pubmed-meshheading:16249329-Enhancer Elements, Genetic,
pubmed-meshheading:16249329-Gene Expression Regulation, Developmental,
pubmed-meshheading:16249329-Genes,
pubmed-meshheading:16249329-Mesoderm,
pubmed-meshheading:16249329-MicroRNAs,
pubmed-meshheading:16249329-Nuclear Proteins,
pubmed-meshheading:16249329-Phosphoproteins,
pubmed-meshheading:16249329-RNA Precursors,
pubmed-meshheading:16249329-Tissue Distribution,
pubmed-meshheading:16249329-Transcription, Genetic,
pubmed-meshheading:16249329-Transcription Factors,
pubmed-meshheading:16249329-Twist Transcription Factor
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pubmed:year |
2005
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pubmed:articleTitle |
Spatial regulation of microRNA gene expression in the Drosophila embryo.
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pubmed:affiliation |
Division of Genetics and Development, Department of Molecular Cell Biology, Center for Integrative Genomics, University of California, Berkeley, CA 94720, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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