Linked Life Data

15930184

Source:http://linkedlifedata.com/resource/pubmed/id/15930184

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2005-6-2
pubmed:abstractText
Steroid receptors are key transcriptional regulators of mammary growth, development and lactation. Expression of estrogen receptors alpha (ERalpha) and beta (ERbeta), progesterone receptor (PR), and estrogen-related receptor alpha-1 (ERRbeta) have been evaluated in bovine mammary gland. The ERRalpha is an orphan receptor that, in other species and tissues, appears to function in the regulation of estrogen-response genes including lactoferrin and medium chain acyl-CoA dehydrogenase and in mitochondrial biogenesis. Expression of ERalpha, ERbeta, PR and ERRalpha was characterized in mammary tissue obtained from multiple stages of bovine mammary gland development using quantitative real-time RT-PCR. Expression was evaluated in prepubertal heifers, primigravid cows, lactating non-pregnant cows, lactating pregnant cows and non-lactating pregnant cows (n=4 to 9 animals/stage). In addition, ERalpha, ERbeta, PR and ERRalpha were mapped to chromosomes 9, 10, 15 and 29 respectively, by linkage and radiation hybrid mapping. Results indicated that expression of ERalpha, PR and ERRalpha was largely coordinately regulated and they were present in significant quantity during all physiological stages evaluated. In contrast, ERbeta transcripts were present at a very low concentration during all stages. Furthermore, no ERbeta protein could be detected in bovine mammary tissue by immunohistochemistry. The ERalpha and PR proteins were detected during all physiological states, including lactation. Our results demonstrate the presence of ERalpha, PR and ERRalpha during all physiological stages, and suggest a functional role for ERRalpha and a relative lack of a role for ERbeta in bovine mammary gland development and lactation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0022-0795
pubmed:author
pubmed:issnType
Print
pubmed:volume
185
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
593-603
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15930184-Animals, pubmed-meshheading:15930184-Cattle, pubmed-meshheading:15930184-Chromosome Mapping, pubmed-meshheading:15930184-Chromosomes, Mammalian, pubmed-meshheading:15930184-Estrogen Receptor alpha, pubmed-meshheading:15930184-Estrogen Receptor beta, pubmed-meshheading:15930184-Female, pubmed-meshheading:15930184-Immunohistochemistry, pubmed-meshheading:15930184-Lactation, pubmed-meshheading:15930184-Mammary Glands, Animal, pubmed-meshheading:15930184-Pituitary Gland, pubmed-meshheading:15930184-Pregnancy, pubmed-meshheading:15930184-Pregnancy, Animal, pubmed-meshheading:15930184-RNA, Messenger, pubmed-meshheading:15930184-Receptors, Cytoplasmic and Nuclear, pubmed-meshheading:15930184-Receptors, Estrogen, pubmed-meshheading:15930184-Receptors, Progesterone, pubmed-meshheading:15930184-Reverse Transcriptase Polymerase Chain Reaction
pubmed:year
2005
pubmed:articleTitle
Chromosomal mapping and quantitative analysis of estrogen-related receptor alpha-1, estrogen receptors alpha and beta and progesterone receptor in the bovine mammary gland.
pubmed:affiliation
Bovine Functional Genomics Laboratory, USDA-ARS, Beltsville, Maryland 20705, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't