Source:http://linkedlifedata.com/resource/pubmed/id/15790784
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2005-6-21
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| pubmed:abstractText |
Exosomes are secreted vesicles formed in late endocytic compartments. Immature dendritic cells (DCs) secrete exosomes, which transfer functional major histocompatibility complex (MHC)-peptide complexes to other DCs. Since immature and mature DCs induce different functional T-cell responses (ie, tolerance versus priming), we asked whether DC maturation also influenced the priming abilities of their exosomes. We show that exosomes secreted by lipopolysaccharide (LPS)-treated mature DCs are 50- to 100-fold more potent to induce antigen-specific T-cell activation in vitro than exosomes from immature DCs. In vitro, exosomes from mature DCs transfer to B lymphocytes the ability to prime naive T cells. In vivo, only mature exosomes trigger effector T-cell responses, leading to fast skin graft rejection. Proteomic and biochemical analyses revealed that mature exosomes are enriched in MHC class II, B7.2, intercellular adhesion molecule 1 (ICAM-1), and bear little milk-fat globule-epidermal growth factor-factor VIII (MFG-E8) as compared with immature exosomes. Functional analysis using DC-derived exosomes from knock-out mice showed that MHC class II and ICAM-1 are required for mature exosomes to prime naive T cells, whereas B7.2 and MFG-E8 are dispensable. Therefore, changes in protein composition and priming abilities of exosomes reflect the maturation signals received by DCs.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD80,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD86,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Cd86 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Adhesion Molecule-1,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Mfge8 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Milk Proteins
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0006-4971
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
1
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| pubmed:volume |
106
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
216-23
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:15790784-Animals,
pubmed-meshheading:15790784-Antigens, CD,
pubmed-meshheading:15790784-Antigens, CD80,
pubmed-meshheading:15790784-Antigens, CD86,
pubmed-meshheading:15790784-Antigens, Surface,
pubmed-meshheading:15790784-CD4-Positive T-Lymphocytes,
pubmed-meshheading:15790784-Cell Communication,
pubmed-meshheading:15790784-Cell Division,
pubmed-meshheading:15790784-Dendritic Cells,
pubmed-meshheading:15790784-Intercellular Adhesion Molecule-1,
pubmed-meshheading:15790784-Lipopolysaccharides,
pubmed-meshheading:15790784-Membrane Glycoproteins,
pubmed-meshheading:15790784-Mice,
pubmed-meshheading:15790784-Mice, Inbred C57BL,
pubmed-meshheading:15790784-Mice, Mutant Strains,
pubmed-meshheading:15790784-Microscopy, Electron,
pubmed-meshheading:15790784-Milk Proteins,
pubmed-meshheading:15790784-Phagocytosis,
pubmed-meshheading:15790784-Secretory Vesicles
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| pubmed:year |
2005
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| pubmed:articleTitle |
ICAM-1 on exosomes from mature dendritic cells is critical for efficient naive T-cell priming.
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| pubmed:affiliation |
Institut National de la Santé et de la Recherche Médicale, Laboratory of Mass Spectrometry and Proteomics, Centre National de la Recherche Scientifique-Unité Mixte de Recherche, Paris, France.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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