Source:http://linkedlifedata.com/resource/pubmed/id/15743320
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2005-3-3
|
| pubmed:abstractText |
The purpose of the present paper was to examine the level of apoptosis and the relationships among apoptosis, apoptosis-associated proteins, and proliferating potential in lymphoma tissues to clarify the characteristics of apoptosis in diffuse large B-cell lymphomas (DLBCL) of the central nervous system (CNS). The formalin-fixed, paraffin-embedded tissues of CNS and non-CNS DLBCL (20 cases each) were studied by terminal deoxynucleotidyl transferase-mediated dUTP-nick end labeling (TUNEL) and immunohistochemistry, using antibodies against single-stranded DNA (ssDNA), cleaved caspase-3, bcl-2, bax, p53, Fas and Ki-67. The cleaved caspase-3 immunohistochemistry detected apoptosis of the lymphoma cells most sensitively compared to TUNEL and ssDNA immunohistochemistry. High expression (grade + + or + + +) of cleaved caspase-3 was found more frequently in CNS DLBCL (11 cases, 55%) than non-CNS DLBCL (three cases, 15%; P = 0.009). Bax-positivity of lymphoma cells was increased in six cases of CNS DLBCL, which also showed high positivity of cleaved caspase-3. There was no significant correlation between the cleaved caspase-3-positivity and the Ki-67 positivity. The present study indicates that the number of apoptotic cells and expression level of cleaved caspase-3 were significantly higher in CNS DLBCL than non-CNS DLBCL, and that the correlation of bax and cleaved caspase-3 expression was often present in CNS DLBCL.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CASP3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Caspases,
http://linkedlifedata.com/resource/pubmed/chemical/Ki-67 Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Markers, Biological
|
| pubmed:status |
MEDLINE
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| pubmed:month |
Mar
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| pubmed:issn |
1320-5463
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
55
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
122-9
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:15743320-Adult,
pubmed-meshheading:15743320-Aged,
pubmed-meshheading:15743320-Aged, 80 and over,
pubmed-meshheading:15743320-Apoptosis,
pubmed-meshheading:15743320-Brain Neoplasms,
pubmed-meshheading:15743320-Caspase 3,
pubmed-meshheading:15743320-Caspases,
pubmed-meshheading:15743320-Combined Modality Therapy,
pubmed-meshheading:15743320-Disease-Free Survival,
pubmed-meshheading:15743320-Female,
pubmed-meshheading:15743320-Humans,
pubmed-meshheading:15743320-Immunoenzyme Techniques,
pubmed-meshheading:15743320-In Situ Nick-End Labeling,
pubmed-meshheading:15743320-Ki-67 Antigen,
pubmed-meshheading:15743320-Lymphoma, B-Cell,
pubmed-meshheading:15743320-Lymphoma, Large B-Cell, Diffuse,
pubmed-meshheading:15743320-Male,
pubmed-meshheading:15743320-Middle Aged,
pubmed-meshheading:15743320-Tumor Markers, Biological
|
| pubmed:year |
2005
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| pubmed:articleTitle |
Immunohistochemical analysis of cleaved caspase-3 detects high level of apoptosis frequently in diffuse large B-cell lymphomas of the central nervous system.
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| pubmed:affiliation |
Department of Human Pathology, Gunma University Graduate School of Medicine, Maebashi, Japan.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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