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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2004-11-2
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pubmed:abstractText |
The IGF-II/M6P receptor targets cathepsin D to the lysosomes and it also binds IGF-II. Although the binding sites for IGF-II and cathepsin D are distinct, reciprocal interactions between the ligands have been observed. We have demonstrated that proIGF-II expression modulates routing of cathepsin D. To test the hypothesis that IGF-II modulation of cathepsin D routing in MCF-7 cells involves IGF-II binding to the IGF-II/M6P receptor, we expressed a mutant form of IGF-II (Arg54 Arg55) that does not bind the IGF-II/M6P receptor and evaluated its effects on cathepsin D secretion. Northern blotting, Western and radioimmunoassay analyses confirmed that these cells express high levels of (Arg54 Arg55) IGF-II mRNA and secretes high levels of IGF-II without modulating the secretion of cathepsin D. These data provide direct evidence that the IGF-II modulation of cathepsin D routing is IGF-II/M6P receptor mediated.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0897-7194
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
169-77
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:15518240-Animals,
pubmed-meshheading:15518240-Arginine,
pubmed-meshheading:15518240-Blotting, Northern,
pubmed-meshheading:15518240-Blotting, Western,
pubmed-meshheading:15518240-Breast Neoplasms,
pubmed-meshheading:15518240-Cathepsin D,
pubmed-meshheading:15518240-Cell Line,
pubmed-meshheading:15518240-Cell Line, Tumor,
pubmed-meshheading:15518240-Culture Media, Conditioned,
pubmed-meshheading:15518240-DNA, Complementary,
pubmed-meshheading:15518240-Dose-Response Relationship, Drug,
pubmed-meshheading:15518240-Genetic Vectors,
pubmed-meshheading:15518240-Humans,
pubmed-meshheading:15518240-Immunoblotting,
pubmed-meshheading:15518240-Immunoprecipitation,
pubmed-meshheading:15518240-Insulin-Like Growth Factor II,
pubmed-meshheading:15518240-Lysosomes,
pubmed-meshheading:15518240-Mice,
pubmed-meshheading:15518240-Microsomes, Liver,
pubmed-meshheading:15518240-RNA, Messenger,
pubmed-meshheading:15518240-Radioimmunoassay,
pubmed-meshheading:15518240-Time Factors,
pubmed-meshheading:15518240-Transfection
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pubmed:year |
2004
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pubmed:articleTitle |
Modulation of cathepsin D routing by IGF-II involves IGF-II binding to IGF-II/M6P receptor in MCF-7 breast cancer cells.
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pubmed:affiliation |
Loma Linda University School of Medicine, Loma Linda, CA 92350, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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