Source:http://linkedlifedata.com/resource/pubmed/id/15031784
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7
|
| pubmed:dateCreated |
2004-3-19
|
| pubmed:abstractText |
Twenty-one cases of acute flaccid paralysis (AFP) were reported on the island of Hispaniola in 2000. Laboratory analysis confirmed the presence of circulating vaccine-derived poliovirus (cVDPV) type 1 in stool samples obtained from patients. As a complement to the active search for cases of AFP, environmental sampling was conducted during November and December 2000, to test for cVDPV in sewage, streams, canals, and public latrines. Fifty-five environmental samples were obtained and analyzed for the presence of polioviruses by use of cell culture followed by neutralization and reverse-transcription polymerase chain reaction. Of the 23 positive samples, 10 tested positive for poliovirus type 1, 7 tested positive for poliovirus type 2, 5 tested positive for poliovirus type 3, and 1 tested positive for both poliovirus type 2 and type 3. By sequence analysis of the complete viral capsid gene 1 (VP1), a 2.1%-3.7% genetic sequence difference between 7 type 1 strains and Sabin type 1 vaccine strain was found. Phylogenetic analysis showed that these viruses are highly related to cVDPV isolated from clinical cases and form distinct subclusters related to geographic region. Our findings demonstrate a useful role for environmental surveillance of neurovirulent polioviruses in the overall polio eradication program.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0022-1899
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| pubmed:author |
pubmed-author:BurnsCaraC,
pubmed-author:CaceresVictor MVM,
pubmed-author:DobbinsJames GJG,
pubmed-author:GaryHoward EHEJr,
pubmed-author:GregoricusNicoleN,
pubmed-author:KewOlenO,
pubmed-author:KoGwangpyoG,
pubmed-author:LandaverdeMauricioM,
pubmed-author:MacadamAndrewA,
pubmed-author:MartinJavierJ,
pubmed-author:SobseyMark DMD,
pubmed-author:VenczelLindaL,
pubmed-author:VinjéJanJ,
pubmed-author:WaitDouglasD
|
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
189
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1168-75
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:15031784-Animals,
pubmed-meshheading:15031784-Disease Outbreaks,
pubmed-meshheading:15031784-Dominican Republic,
pubmed-meshheading:15031784-Female,
pubmed-meshheading:15031784-Haiti,
pubmed-meshheading:15031784-Humans,
pubmed-meshheading:15031784-Male,
pubmed-meshheading:15031784-Mice,
pubmed-meshheading:15031784-Mice, Transgenic,
pubmed-meshheading:15031784-Neutralization Tests,
pubmed-meshheading:15031784-Poliomyelitis,
pubmed-meshheading:15031784-Poliovirus,
pubmed-meshheading:15031784-Poliovirus Vaccines,
pubmed-meshheading:15031784-Prevalence,
pubmed-meshheading:15031784-RNA, Viral,
pubmed-meshheading:15031784-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15031784-Sewage,
pubmed-meshheading:15031784-Viral Nonstructural Proteins,
pubmed-meshheading:15031784-Water Microbiology
|
| pubmed:year |
2004
|
| pubmed:articleTitle |
Isolation and characterization of circulating type 1 vaccine-derived poliovirus from sewage and stream waters in Hispaniola.
|
| pubmed:affiliation |
Department of Environmental Sciences and Engineering, School of Public Health, University of North Carolina, Chapel Hill, North Carolina 27599-7431, USA. janvinje@email.unc.edu.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|