Linked Life Data

14704722

Source:http://linkedlifedata.com/resource/pubmed/id/14704722

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2004-3-15
pubmed:abstractText
Chronic gastritis is frequently associated with infection of Helicobacter pylori and characterized by tissue infiltration of neutrophils, lymphocytes, and plasma cells. To address the mechanism of lymphocyte infiltration in chronic gastritis, we examined the expression of chemokines and their receptors using frozen sections of chronic gastritis, obtained from 23 patients who underwent gastrectomy for gastric cancer. By immunohistochemistry, lymphocytes in inflamed gastric mucosa expressed CCR5 abundantly, CXCR3 less frequently, and CCR4 sparsely. The numbers of CCR5(+) cells, which were composed of mainly CD8(+) and partly CD4(+) T cells, were positively correlated with the degree of neutrophil infiltration, and decreased in areas with intestinal metaplasia or mucosal atrophy. RANTES/CCL5, one of the ligands of CCR5, was localized mainly in CD8(+) and partly CD4(+) T cells with a characteristic dotted pattern, and such lymphocytes were most densely distributed around the neck region of gastric glands. In situ hybridization confirmed the expression of CCL5 mRNA in these cells, and immunoelectron microscopy revealed localization of CCL5 in the membrane-bound granules, which most probably corresponded to the cytolytic granules of cytotoxic T cells. The numbers of CCL5(+) lymphocytes showed a close correlation with the degree of neutrophil infiltration and markedly decreased in intestinal metaplasia. In conclusion, our data suggest that, together with neutrophils, CCL5(+) T cells, presumably activated cytotoxic T cells, would play important roles in the active inflammatory process of chronic gastritis. Our data also suggest a self-recruiting mechanism involving CCR5 and CCL5 for tissue accumulation of such T cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0023-6837
pubmed:author
pubmed:issnType
Print
pubmed:volume
84
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
368-75
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:14704722-Adult, pubmed-meshheading:14704722-Aged, pubmed-meshheading:14704722-Aged, 80 and over, pubmed-meshheading:14704722-CD8-Positive T-Lymphocytes, pubmed-meshheading:14704722-Chemokine CCL5, pubmed-meshheading:14704722-Chemokines, pubmed-meshheading:14704722-Chemokines, CC, pubmed-meshheading:14704722-Chronic Disease, pubmed-meshheading:14704722-Cytokines, pubmed-meshheading:14704722-Cytoplasmic Granules, pubmed-meshheading:14704722-Female, pubmed-meshheading:14704722-Gastritis, pubmed-meshheading:14704722-Humans, pubmed-meshheading:14704722-Immunohistochemistry, pubmed-meshheading:14704722-In Situ Hybridization, pubmed-meshheading:14704722-Male, pubmed-meshheading:14704722-Microscopy, Immunoelectron, pubmed-meshheading:14704722-Middle Aged, pubmed-meshheading:14704722-Receptors, Chemokine, pubmed-meshheading:14704722-Reverse Transcriptase Polymerase Chain Reaction
pubmed:year
2004
pubmed:articleTitle
Infiltration of CD8+ T cells containing RANTES/CCL5+ cytoplasmic granules in actively inflammatory lesions of human chronic gastritis.
pubmed:affiliation
Department of Pathology, Tohoku University Graduate School of Medicine, Japan.
pubmed:publicationType
Journal Article