rdf:type |
|
lifeskim:mentions |
umls-concept:C0007447,
umls-concept:C0012550,
umls-concept:C0040715,
umls-concept:C0205160,
umls-concept:C0439799,
umls-concept:C0559956,
umls-concept:C0596901,
umls-concept:C0599718,
umls-concept:C0599813,
umls-concept:C0599893,
umls-concept:C1280500,
umls-concept:C1522492,
umls-concept:C1522702,
umls-concept:C1708096,
umls-concept:C2825490
|
pubmed:issue |
17
|
pubmed:dateCreated |
1992-7-16
|
pubmed:abstractText |
Diphtheria toxin B fragment is capable of forming cation-selective channels in the plasma membrane. Such channels may be involved in the translocation of the toxin A fragment to the cytosol. Seven negatively charged amino acids in the B fragment were replaced one by one by lysines, followed by studies of cytotoxicity and channel-forming ability of the different mutants. The mutant D392K showed a strong reduction in binding to cell surface receptors. Of the six mutants that showed wild-type binding affinity, the two mutants D295K and D318K were very inefficient in forming channels. These two mutants had the lowest ability to mediate A fragment translocation. The mutant E362K was able both to induce cation channel formation and to mediate A fragment translocation at a higher pH value than the wild-type B fragment. The results support the notion that formation of cation channels is of importance for the translocation of the A fragment across the plasma membrane, and they indicate that the pH requirement for translocation of the A fragment to the cytosol is partly determined by the B fragment.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Jun
|
pubmed:issn |
0021-9258
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:day |
15
|
pubmed:volume |
267
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
12284-90
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:1376320-Amino Acid Sequence,
pubmed-meshheading:1376320-Ammonium Chloride,
pubmed-meshheading:1376320-Animals,
pubmed-meshheading:1376320-Base Sequence,
pubmed-meshheading:1376320-Cations,
pubmed-meshheading:1376320-Cell Membrane,
pubmed-meshheading:1376320-Cell Survival,
pubmed-meshheading:1376320-Diphtheria Toxin,
pubmed-meshheading:1376320-Electrochemistry,
pubmed-meshheading:1376320-Endopeptidase K,
pubmed-meshheading:1376320-Hydrogen-Ion Concentration,
pubmed-meshheading:1376320-Ion Channels,
pubmed-meshheading:1376320-Molecular Sequence Data,
pubmed-meshheading:1376320-Mutagenesis, Insertional,
pubmed-meshheading:1376320-Peptide Fragments,
pubmed-meshheading:1376320-Serine Endopeptidases,
pubmed-meshheading:1376320-Sodium,
pubmed-meshheading:1376320-Vero Cells
|
pubmed:year |
1992
|
pubmed:articleTitle |
Replacement of negative by positive charges in the presumed membrane-inserted part of diphtheria toxin B fragment. Effect on membrane translocation and on formation of cation channels.
|
pubmed:affiliation |
Institute for Cancer Research, Norwegian Radium Hospital, Oslo, Norway.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|