Linked Life Data

1373165

Source:http://linkedlifedata.com/resource/pubmed/id/1373165

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1992-5-13
pubmed:abstractText
A variety of adhesion molecules regulate the traffic and tissue localization of lymphocytes in vivo by mediating their binding to vascular endothelial cells. The homing receptor gp90MEL-14 (gp90), also known as LECAM-1 or L-selectin, mediates the adhesion of lymphocytes to specialized high endothelial venules in lymph nodes (LN) and is the primary molecule regulating lymphocyte recirculation and homing to LN, whereas other adhesion molecules have a major role in the localization of lymphocytes in inflammatory sites. We used four-color flow cytometric analysis to examine the regulation of adhesion receptor expression on LN CD8 T cells responding to skin allografts in vivo. In normal mice, greater than 95% of LN CD8 T cells are gp90+, being either gp90+Pgp1- (Population (Pop.) 1 or gp90+Pgp-1+ (Pop.2). Allografting induces the down-regulation of gp90 and up-regulation of Pgp-1 on a subset of cells, resulting in the appearance of CD8+gp90-Pgp-1hi (Pop. 3) cells. Pop. 3 cells also express high levels of LFA-1, ICAM-1, and ICAM-2, and a subset of them are VLA-4 alpha-positive. Purified Pop. 3 cells have potent cytolytic activity directed against donor alloantigen, whereas no such activity is present in Pop. 1 or 2 cells. Correlating with this is the high granzyme activity in Pop. 3 cells. In addition, Pop. 3 lymphocytes, but not Pop. 1 or 2, secrete a large amount of IFN-gamma in response to Ag. Finally, the CD8 T cells that infiltrate sponge matrix allografts are markedly enriched for the Pop. 3 subset. These results show that, during the immune response to alloantigen in vivo, a small subset of CD8 T cells down-regulates the LN homing receptor while increasing the expression of other adhesion molecules, as they differentiate into highly active cytolytic T lymphocytes. Thus, the differential regulation of LN homing receptors and receptors for peripheral vascular endothelium provides a mechanism that would redirect the traffic of activated effector cells away from lymphoid tissue and to sites of Ag deposition, where they would participate in the inflammatory response.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
148
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2348-56
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:1373165-Animals, pubmed-meshheading:1373165-Antigens, CD8, pubmed-meshheading:1373165-Cell Adhesion, pubmed-meshheading:1373165-Cell Adhesion Molecules, pubmed-meshheading:1373165-Cell Differentiation, pubmed-meshheading:1373165-Immunologic Memory, pubmed-meshheading:1373165-Isoantigens, pubmed-meshheading:1373165-L-Selectin, pubmed-meshheading:1373165-Lymphocyte Function-Associated Antigen-1, pubmed-meshheading:1373165-Mice, pubmed-meshheading:1373165-Mice, Inbred BALB C, pubmed-meshheading:1373165-Mice, Inbred C57BL, pubmed-meshheading:1373165-Phenotype, pubmed-meshheading:1373165-Receptors, Lymphocyte Homing, pubmed-meshheading:1373165-Receptors, Very Late Antigen, pubmed-meshheading:1373165-Skin Transplantation, pubmed-meshheading:1373165-T-Lymphocytes, Cytotoxic, pubmed-meshheading:1373165-Transplantation, Homologous
pubmed:year
1992
pubmed:articleTitle
Regulation of adhesion molecule expression by CD8 T cells in vivo. I. Differential regulation of gp90MEL-14 (LECAM-1), Pgp-1, LFA-1, and VLA-4 alpha during the differentiation of cytotoxic T lymphocytes induced by allografts.
pubmed:affiliation
Department of Pathology, University of Iowa College of Medicine, Iowa City 52242.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.