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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1992-2-14
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pubmed:databankReference |
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pubmed:abstractText |
A number of neuropeptides have been described which are present in the insect nervous system. The physiological role of these neuropeptides has not yet been clarified. We have characterized a Drosophila melanogaster cDNA coding for a protein, NKD, whose sequence resembles that of mammalian G protein-coupled neuropeptide receptors. This protein shows 38% homology with the mammalian tachykinin NK3 receptor within the transmembrane domain region. Stable cell lines expressing this cDNA are responsive to Locusta migratoria tachykinin but not to other peptides of the tachykinin family. The expression of this gene is detected principally in adult fly heads, but also in the adult body and in embryos. Interestingly, NKD mRNA is detected at very early stages of Drosophila embryonic development (3 h) and reaches the highest level of expression at 12-16 h, a time which correlates with the period of major neuronal development. In situ hybridization experiments demonstrate that NKD is expressed in the central nervous system, as well as in subsets of neurons in each segment of the developing ventral ganglia. The cytological localization of this gene is at position 86C on the Drosophila third chromosome.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
267
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1298-302
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1370464-3T3 Cells,
pubmed-meshheading:1370464-Amino Acid Sequence,
pubmed-meshheading:1370464-Animals,
pubmed-meshheading:1370464-Blotting, Northern,
pubmed-meshheading:1370464-Cell Line, Transformed,
pubmed-meshheading:1370464-DNA,
pubmed-meshheading:1370464-Drosophila Proteins,
pubmed-meshheading:1370464-Drosophila melanogaster,
pubmed-meshheading:1370464-Gene Expression Regulation,
pubmed-meshheading:1370464-Genetic Vectors,
pubmed-meshheading:1370464-Mice,
pubmed-meshheading:1370464-Molecular Sequence Data,
pubmed-meshheading:1370464-RNA,
pubmed-meshheading:1370464-Receptors, Neurotransmitter,
pubmed-meshheading:1370464-Receptors, Tachykinin,
pubmed-meshheading:1370464-Sequence Alignment
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pubmed:year |
1992
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pubmed:articleTitle |
NKD, a developmentally regulated tachykinin receptor in Drosophila.
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pubmed:affiliation |
Laboratoire de Génétique des Eucaryotes, Centre National de la Recherche Scientifique, l'Institut National de la Santé et de la Recherche Médicale, Faculté de Médecine de Strasbourg, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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