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rdf:type |
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lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0033684,
umls-concept:C0086418,
umls-concept:C0449205,
umls-concept:C0971285,
umls-concept:C1513354,
umls-concept:C1514873,
umls-concept:C1538613,
umls-concept:C1546857,
umls-concept:C1556066,
umls-concept:C1619636
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pubmed:issue |
5
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pubmed:dateCreated |
2003-9-18
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pubmed:databankReference |
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pubmed:abstractText |
Aurora family kinases contribute to regulation of mitosis. Using RNA interference in synchronized HeLa cells, we now show that Aurora-A is required for mitotic entry. We found that initial activation of Aurora-A in late G2 phase of the cell cycle is essential for recruitment of the cyclin B1-Cdk1 complex to centrosomes, where it becomes activated and commits cells to mitosis. A two-hybrid screen identified the LIM protein Ajuba as an Aurora-A binding protein. Ajuba and Aurora-A interact in mitotic cells and become phosphorylated as they do so. In vitro analyses revealed that Ajuba induces the autophosphorylation and consequent activation of Aurora-A. Depletion of Ajuba prevented activation of Aurora-A at centrosomes in late G2 phase and inhibited mitotic entry. Overall, our data suggest that Ajuba is an essential activator of Aurora-A in mitotic commitment.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CCNB1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/CDC2 Protein Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/Ccnb1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin B,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin B1,
http://linkedlifedata.com/resource/pubmed/chemical/Eg2 protein, Xenopus,
http://linkedlifedata.com/resource/pubmed/chemical/Glutathione Transferase,
http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/JUB protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Jub protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Xenopus Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/aurora kinase
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
114
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
585-98
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pubmed:dateRevised |
2011-7-11
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pubmed:meshHeading |
pubmed-meshheading:13678582-Animals,
pubmed-meshheading:13678582-CDC2 Protein Kinase,
pubmed-meshheading:13678582-COS Cells,
pubmed-meshheading:13678582-Cell Cycle,
pubmed-meshheading:13678582-Cell Cycle Proteins,
pubmed-meshheading:13678582-Cell Line,
pubmed-meshheading:13678582-Centrosome,
pubmed-meshheading:13678582-Cyclin B,
pubmed-meshheading:13678582-Cyclin B1,
pubmed-meshheading:13678582-G2 Phase,
pubmed-meshheading:13678582-Gene Deletion,
pubmed-meshheading:13678582-Glutathione Transferase,
pubmed-meshheading:13678582-HeLa Cells,
pubmed-meshheading:13678582-Homeodomain Proteins,
pubmed-meshheading:13678582-Humans,
pubmed-meshheading:13678582-Microscopy, Fluorescence,
pubmed-meshheading:13678582-Mitosis,
pubmed-meshheading:13678582-Models, Biological,
pubmed-meshheading:13678582-Molecular Sequence Data,
pubmed-meshheading:13678582-Phosphorylation,
pubmed-meshheading:13678582-Precipitin Tests,
pubmed-meshheading:13678582-Protein Binding,
pubmed-meshheading:13678582-Protein Kinases,
pubmed-meshheading:13678582-Protein-Serine-Threonine Kinases,
pubmed-meshheading:13678582-RNA Interference,
pubmed-meshheading:13678582-Recombinant Proteins,
pubmed-meshheading:13678582-Temperature,
pubmed-meshheading:13678582-Time Factors,
pubmed-meshheading:13678582-Two-Hybrid System Techniques,
pubmed-meshheading:13678582-Xenopus Proteins
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pubmed:year |
2003
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pubmed:articleTitle |
Aurora-A and an interacting activator, the LIM protein Ajuba, are required for mitotic commitment in human cells.
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pubmed:affiliation |
Department of Tumor Genetics and Biology, Graduate School of Medical Sciences, Kumamoto University, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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