Linked Life Data

1353788

Source:http://linkedlifedata.com/resource/pubmed/id/1353788

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1992-9-9
pubmed:abstractText
The distribution of inhibitor-1, a cyclic AMP-regulated inhibitor of protein phosphatase-1, was analyzed in various brain regions and peripheral tissues of various species by immunolabeling of sodium dodecyl sulfate-poly-acrylamide gel transfers using specific antibodies. The distribution of inhibitor-1 was directly compared to that of DARPP-32, a structurally related cyclic AMP-regulated inhibitor of protein phosphatase-1. In rat CNS, a single immunoreactive protein of M(r) 30,000, identified as inhibitor-1, was widely distributed. In contrast, DARPP-32 was highly concentrated in the basal ganglia. Inhibitor-1 was detected in brain tissue from frog (M(r) 27,000), turtle (M(r) 29,000/33,000), canary (M(r) 26,000), pigeon (M(r) 28,000), mouse (M(r) 30,500), rabbit (M(r) 26,500), cow (M(r) 27,000), and monkey (M(r) 27,500), but not from goldfish. Inhibitor-1 was detected at various levels in most peripheral tissues of the species studied; however, it was not detectable in certain tissues of particular species (e.g., rat and cow liver). DARPP-32 was detected in brain tissue of all the species tested except frog and goldfish, but was not detectable in most peripheral tissues. Both inhibitor-1 and DARPP-32 were concentrated in the cytosol and synaptosomal cytosol of rat striatum. The developmental expressions of inhibitor-1 and DARPP-32 in rat striatum differed: the level of inhibitor-1 peaked in the first postnatal week and then declined by the third postnatal week, whereas the level of DARPP-32 increased to a peak level by the third postnatal week and remained elevated thereafter.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0022-3042
pubmed:author
pubmed:issnType
Print
pubmed:volume
59
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1053-61
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:1353788-Animals, pubmed-meshheading:1353788-Brain, pubmed-meshheading:1353788-Carrier Proteins, pubmed-meshheading:1353788-Central Nervous System, pubmed-meshheading:1353788-Dopamine and cAMP-Regulated Phosphoprotein 32, pubmed-meshheading:1353788-Enzyme Inhibitors, pubmed-meshheading:1353788-Female, pubmed-meshheading:1353788-Intracellular Signaling Peptides and Proteins, pubmed-meshheading:1353788-Male, pubmed-meshheading:1353788-Nerve Tissue Proteins, pubmed-meshheading:1353788-Phosphoproteins, pubmed-meshheading:1353788-Proteins, pubmed-meshheading:1353788-Rats, pubmed-meshheading:1353788-Rats, Inbred Strains, pubmed-meshheading:1353788-Subcellular Fractions, pubmed-meshheading:1353788-Tissue Distribution, pubmed-meshheading:1353788-Tyrosine 3-Monooxygenase, pubmed-meshheading:1353788-Vertebrates
pubmed:year
1992
pubmed:articleTitle
Distribution of protein phosphatase inhibitor-1 in brain and peripheral tissues of various species: comparison with DARPP-32.
pubmed:affiliation
Laboratory of Molecular and Cellular Neuroscience, Rockefeller University, New York, New York 10021-6399.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.