Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1992-4-6
pubmed:abstractText
The synthesis and expression of voltage-dependent sodium (Na) channels is a crucial aspect of neuronal differentiation because of the central role these ion channels play in the generation of action potentials and the transfer of information in the nervous system. We have used rat pheochromocytoma (PC12) cell lines deficient in cAMP-dependent protein kinase (PKA) activity to examine the role of PKA in the induction of Na channel expression by nerve growth factor (NGF) and basic FGF (bFGF). In the parental PC12 cell line both NGF and bFGF elicit an increase in the density of functional Na channels, as determined from whole-cell patch clamp recordings. This increase does not occur in two PC12 cell lines deficient in both isozymes of PKA (PKAI and PKAII), and is strongly reduced in a third line deficient in PKAII, but not PKAI. Despite the inability of the neurotrophic factors to induce functional Na channel expression in the PKA-deficient cells, Northern blot hybridization studies and saxitoxin binding assays of intact cells indicate that NGF and bFGF are still capable of eliciting increases in both Na channel mRNA and Na channel protein in the membrane. Thus, PKA activity appears to be necessary at a posttranslational step in the synthesis and expression of functional Na channels, and thereby plays an important role in determining neuronal excitability.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-1065897, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-12991237, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-1651325, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-1689374, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-1690563, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-1722983, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-1849906, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-1851699, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-1908468, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-2032465, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-2032466, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-2099797, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-2155009, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-2157719, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-2167354, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-2302564, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-2410908, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-2425982, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-2427371, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-2442166, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-2446328, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-2447073, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-2448784, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-2449363, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-2449364, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-2452597, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-2479727, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-2540698, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-2543677, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-2546952, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-2555170, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-2560648, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-2647474, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-2682327, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-2856097, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-2856102, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-3018542, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-3285771, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-329148, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-3301324, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-4047401, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-518835, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-6159658, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-6270629, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-6282861, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-6292214, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-6319405, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-7119865, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311713-999827
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0021-9525
pubmed:author
pubmed:issnType
Print
pubmed:volume
116
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1465-73
pubmed:dateRevised
2010-9-7
pubmed:meshHeading
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